人白血病抑制因子真核表达载体的构建及在哺乳动物细胞中的表达  

Construction of eukaryotic expression vector of human leukemia inhibitory factor and its expression in mammalian cells

作　　者：马涛[1] 肖雪[2] 吕琦[2] 王泽华[2] 马岚[2] 

机构地区：[1]中国林业科学研究院资源昆虫研究所,云南昆明650224 [2]云南大学单克隆抗体工程技术中心,云南昆明650091

出　　处：《云南大学学报（自然科学版）》2006年第S1期387-392,395,共7页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金预攀登项目(980174)

摘　　要：应用RT-PCR方法从人子宫内膜组织总RNA扩增出hLIF的全长基因,然后将其克隆至pcDNA3上,成功构建了重组真核表达载体pcDNA3/hLIF.利用脂质体介导将这一表达载体导入COS-7细胞和CHO-K1细胞,分别获得了hLIF的瞬时表达和稳定表达,为进一步进行hLIF生物学功能研究和hLIF在哺乳动物细胞中的高表达研究奠定了基础.The whole gene encoding hLIF protein was amplified from totahis gene was cloned into pcDNA3 vector and constructed the pcDNA3/hLIF expression vector.And finally,pcDNA3/hLIF was introduced into COS-7 cells and CHO-K1 cells by using the method of lipofectin transfection,and transient expression and stable expression of hLIF protein was obtained respectively.These results provide bases for further researches for biological function of hLIF and its high expression in mammalian cells.

关 键 词：人白血病抑制因子 COS-7细胞 CHO-K1细胞 克隆 基因表达 

分 类 号：Q78[生物学—分子生物学]