无柄金丝桃的化学成分研究  

Studies on the chemical constituents of Hypericum augustinii

作　　者：张燕[1] 罗蕾[2] 潘争红[1] 马国义[3] 李祖强[1] 

机构地区：[1]云南大学实验中心,云南昆明650091 [2]云南师范大学化学化工学院,云南昆明650092 [3]悉尼大学药学系

出　　处：《云南大学学报（自然科学版）》2006年第S1期232-236,共5页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(29962004)

摘　　要：采用HL60细胞系对无柄金丝桃(Hypericum augustinii N.Robson)全株的粗提物及其分步萃取物进行了细胞毒试验,结果表明,无柄金丝桃的氯仿及乙酸乙酯提取物有一定细胞毒活性,其IC50值分别为8.0,12.5μg/mL.在细胞毒试验结果指导下,对无柄金丝桃全株的有效部位进行了化学成分分离.首次从该植物中得到7个化合物,通过理化数据测定及波谱数据分析,分别鉴定为1,5,6-三羟基口山酮(1);5-羟基-2-甲氧基口山酮(2);Isojacareubin(3);Hyperibone B(4);Hyperibone E(5);槲皮素(6);木犀草素(7).Cytotoxicity screening(HL60) of extracts of Hypericum augustinii N.Robson was carried out.In the result,CHCl_3 and EtOAc fractions possess cytotoxic activities with IC_(50) 8.0 and 12.5?μg/mL respectively.Seven constituents were isolated from the active fractions of H.augustinii.Their structures were identified by physico-chemical properties and spectral data analysis as 1,5,6-trihydroxyxanthone(1),5-hydroxy-2-methoxyxanthone(2),isojacareubin(3),hyperibone B(4),hyperibone E(5),quercetin(6) and luteolin(7).A...

关 键 词：金丝桃属 无柄金丝桃 化学成分 细胞毒性 

分 类 号：R284[医药卫生—中药学]