水稻腥黑粉病菌的单孢检测  

The detection for single teliospore of Tilletia horrida

作　　者：周业琴[1] 刘素萍[2] 周国梁[3] 易建平[3] 印丽萍[3] 董英[1] 

机构地区：[1]江苏大学,镇江212013 [2]华南农业大学 [3]上海出入境检验检疫局

出　　处：《植物检疫》2006年第S1期38-41,共4页Plant Quarantine

基　　金：上海市重大科技攻关项目(03DZ19315和04DZ05002)资助本项目

摘　　要：根据水稻腥黑粉菌两个聚类群与其它黑粉菌ITS序列的差异,分别设计了两个聚类群的特异引物Hor2/Hor9和Hm1/Hm5,结合ITS通用引物Til1/Til4建立了分别检测水稻腥黑粉菌两个聚类群单个冬孢子的套式(巢式)PCR检测方法,整个检测过程可以缩短至8h。Isolates of Tilletia horrida from different locations were divided into two distinct clades(groups) based on the rDNA ITS sequences,with the sequence difference,the nested PCR methods using group-specific primers Til1/Til4(Hor2/Hor9) and Til1/Til4(Hm1/Hm5) were developed for detecting teliospore of two groups, respectively. One teliospore can be amplified and obtained specific amplicon. The process of detection can be finished within 8 hours. Because of avoidance of DNA extraction,this method can be useful ...

关 键 词：水稻腥黑粉菌 套式(巢式)PCR 冬孢子 检测 

分 类 号：S41-30[农业科学—植物保护]