利用实时荧光RT-PCR方法检测番茄环斑病毒和烟草环斑病毒  

Detections of Tomato ring spot virus and Tobacco ring spot virus by real-time fluorescent RT-PCR

作　　者：杨翠云[1] 于翠[1] 张舒亚[1] 高焕利[2] 

机构地区：[1]上海出入境检验检疫局,200135 [2]西北农林科技大学

出　　处：《植物检疫》2006年第S1期31-34,共4页Plant Quarantine

摘　　要：根据番茄环斑病毒和烟草环斑病毒各分离物CP基因的保守序列分别设计并合成1对引物和1条TaqMan荧光探针,分别建立了ToRSV和TRSV的实时荧光RT-PCR检测方法。该方法具有更加灵敏、特异、快速和简便的特点,在口岸病害检疫中具有广泛的应用前景。A pair of primers and a TaqMan probe based on the conserved nucleotide sequence of CP gene of several ToRSV and TRSV isolates were designed and synthesized separately. The real-time fluorescent RT-PCR were established separately to detect ToRSV and TRSV. The more sensitive, special, quick and easy-handling detection methods are suitable for diseased quarantine detections.

关 键 词：番茄环斑病毒 烟草环斑病毒 实时荧光RT-PCR 检测方法 

分 类 号：S41-30[农业科学—植物保护]