瑞香狼毒素对菜粉蝶幼虫的拒食活性及其作用机制  

Study on the Antifeedant Activity and Mechanism for Bioactive Compound from Stellera chamaejasme against Larvae of Pieris rapae

作　　者：何义国[1] 张可君[1] 肖波[1] 侯太平[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《中国生物防治》2006年第S1期33-37,共5页Chinese Journal of Biological Control

基　　金：国家自然科学基金(31071072)

摘　　要：瑞香狼毒素1,5-二苯基-3-羟基-1-戊酮,是本室从杀虫植物瑞香狼毒中分离得到的一个高活性化合物。本文研究了该活性成分对菜粉蝶幼虫的拒食作用和对乙酰胆碱酯酶(AChE)活性的影响。采用浸渍叶碟饲喂法测得:在48h时瑞香狼毒素对3龄幼虫的非选择性和选择性拒食作用的拒食中浓度(AFC50)分别为15.1mg/L和16.9mg/L。采用点滴法测得瑞香狼毒素对5龄幼虫的拒食作用的拒食中量(AFD50)为0.565μg/头。瑞香狼毒素对AChE活性的抵制率高达78.5%,表明该活性成分可显著抑制AChE活性。A high bioactive compound 1,5-diphenyl-3-hydroxyl-1-pentanone was isolated from the extract of the roots of Stellera chamaejasme L.The antifeedant activity of the compoud was tested against larvae of Pieris rapae by leaf dipping method and topical application method.The results showed that the AFC_(50) of non-choice and choice antifeeding effects in leaf-dipping test against third instar larvae were 15.1mg/L and 16.9mg/L respectively in 48 hours,and the AFD_(50) of non-choice antifeeding effect by topical a...

关 键 词：瑞香狼毒 1 5-二苯基-3-羟基-1-戊酮 菜粉蝶 拒食作用 乙酰胆碱酯酶 

分 类 号：S482.3[农业科学—农药学]