蛋白质组学在农业中的应用  

Proteomics and its Application in the Agriculture

作　　者：柳展基[1] 杨小红[2] 毕玉平[1] 

机构地区：[1]山东省农科院高新技术研究中心,济南250100 [2]中国农业大学国家玉米改良中心,北京100094

出　　处：《分子植物育种》2006年第z1期106-110,共5页Molecular Plant Breeding

基　　金：国家自然基金(30571165)资助.

摘　　要：随着拟南芥、水稻等模式植物基因组测序的完成,植物基因组学的研究重点已经转变为功能基因组学研究。蛋白质组学是后基因组时代——功能基因组学研究的新兴学科和热点领域。本文简要介绍了蛋白质组学产生的背景、蛋白质组学的含义和研究方法。研究方法主要包括以双向聚丙烯酰胺凝胶电泳为主的蛋白分离技术(2-DE)和以质谱(Mass-spectrometry)分析为主的蛋白鉴定技术。本文详细评述了蛋白质组学技术在农业科学研究中的应用,如核不育和细胞质雄性研究,病虫害等生物胁迫蛋白质组学研究,缺氧胁迫、热胁迫、损伤胁迫等非生物胁迫研究、各种突变体研究、组织和细胞器(叶绿体、线粒体等)蛋白质组研究等。最后展望了蛋白质组学在农业科学研究中的应用前景。With the completeness of the two model plants, Arabidopsis and rice, genomic sequencing project, the emphasis of plant genomic research has focused on functional genome research. Proteomics is one of the most active research fields in the post-genomic era. In this review, we introduced the background of proteomics, definition and the research technologies of proteomics. Two-dimensional electrophoresis (2-DE) and mass spectrometry (MS) are the two key technologies. Moreover, we systemically discussed the application of proteomics in agricultural research, especially in crop science. In the sterility research of crops, proteomic technologies help to find differentially expressed proteins associated with sterility. Proteomic studies are helpful in understanding the response of plants to biotic and abiotic stress. Proteomic differences also exist among organelles in plant cells, such as chloroplast, mitochondrion. Finally, we prospected the proteomic study in the agricultural science.

关 键 词：蛋白质组学 双向电泳 质谱 农业 

分 类 号：S184[农业科学—农业基础科学]