Differential analysis of two-dimensional gel electrophoresis profiles of spermatozoa protein in human normal motility sperm and idiopathic asthenospermia  

作　　者：申树林 贺大林 罗勇 宁亮 

机构地区：[1]Department of Urology,First Affiliated Hospital,Medical School,Xi'an Jiaotong University

出　　处：《Journal of Medical Colleges of PLA(China)》2006年第5期326-329,共4页中国人民解放军军医大学学报（英文版）

摘　　要：Objective: To evaluate the application of two-dimensional electrophoresis in the research of differentially expressed proteins in the human asthenospermia. Methods: Two-dimensional gel electrophoresis was performed on 4 normal sperm samples from healthy men and 4 sperm samples from 4 asthenospermia patients. After silver staining, the differential expression proteins were analyzed by PDQuest 2D analysis software. Results: Six differential protein spots were identified. Four spots showed increased expression in the control gels compared with the patient gels. Conclusion: The protein profiles of differential expression between the normal spermatozoa and idiopathic asthenospermia were established and some differential proteins were found. The data of this study would establish the better fundament for further isolation and identification of differentially expressed proteins in human asthenospermia sperm.Objective: To evaluate the application of two-dimensional electrophoresis inthe research of differentially expressed proteins in the human asthenospermia. Methods:Two-dimensional gel electrophoresis was performed on 4 normal sperm samples from healthy men and 4sperm samples from 4 asthenospermia patients. After silver staining, the differential expressionproteins were analyzed by PDQuest 2D analysis software. Results: Six differential protein spots wereidentified. Four spots showed increased expression in the control gels compared with the patientgels. Conclusion: The protein profiles of differential expression between the normal spermatozoa andidiopathic asthenospermia were established and some differential proteins were found. The data ofthis study would establish the better fundament for further isolation and identification ofdifferentially expressed proteins in human asthenospermia sperm.

关 键 词：IDIOPATHIC ASTHENOSPERMIA PROTEIN two-dimensional gel electrophoresis 

分 类 号：R698[医药卫生—泌尿科学]