抑肽酶对大鼠血肿周围脑组织炎症反应和细胞凋亡的影响  

Effect of aprotinin on inflammation and apoptosis around perihematoma tissue after intracerebral hemorrhage in rats

作　　者：杨德刚[1] 俞龙[1] 徐耀[1] 吴健[1] 袁成林[1] 卢年芳[1] 胡长林[2] 

机构地区：[1]扬州大学医学院附属医院神经内科,江苏扬州225001 [2]重庆医科大学第二附属医院,重庆400010

出　　处：《中风与神经疾病杂志》2006年第5期597-599,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的通过抑肽酶干预,研究大鼠血肿周围脑组织炎症和细胞凋亡的关系,以探讨脑出血后继发性损伤的机制。方法采用立体定向手术脑尾状核胶原酶注射诱导大鼠脑出血模型,用抑肽酶进行干预,于不同时相点测定TUNEL阳性细胞,用组织病理学HE染色观察不同时相点的炎症浸润情况,用免疫组织化学测定IL-1β的蛋白表达水平。结果模型组TUNEL阳性细胞造模后6h即出现,24h明显增加,3d达到高峰,7d时仍有较多表达;HE染色见6h血肿内及周围有少量散在白细胞,24h明显增加,48h到高峰,3d时稍减少,7d时仍有较多的白细胞浸润。模型组血肿周围脑组织的IL-1β阳性细胞数在6h即有增加,48h左右达高峰;抑肽酶组与模型组比较,TUNEL阳性细胞在24h、48h、72h时明显减少(P<0.01);HE染色可见浸润白细胞数目在24h、48h、72h时明显减少(P<0.01)。抑肽酶组IL-1β相应各时间点的阳性表达明显减少,尤以48h、72h较为明显。结论抑肽酶能明显抑制脑出血后的炎症反应和细胞凋亡;而抑制炎症反应很可能是减少脑出血神经细胞凋亡的有效途径之一。Objective To investigate the relationship between inflammation and apoptosis around perihematoma tissue(PHT) after intracerebral hemorrhage (ICH) in rats by aprotinin intervention and explore the secondary damage mechanism after ICH. Methods Intracerebral hemorrhagic model was induced utilizing the local injection of collagenase into the basal ganglia with sterile. In different times,apoptosis levels in PHT was detected by TUNEL technique,the level of inflammation by HE and the interleukin-1β protein expression by immunohistochemistry. Results In model group(MG) TUNEL positive cell in PHT appeared 6h after ICH,increased markedly at 24h,reached the peak on 3d,still remained some on 7d. In MG,neutrophil infiltration increased slightly at 6h,markedly at 24h,reached the maximum at 48h,then decreased slightly at 72h. In MG interleukin-1β expression began to increase at 6h after ICH,reached the peak at 48h. In aprotinin-treated group(ATG),TUNEL positive cells dicrease markly at 24,48,72h(compare with MG P< 0.01 ),and there was a significant decrease of neutrophil infiltration at 24,48,72,168h (compare with MG,P< 0.01 ). Aprotinin reduced the il-1β positive expression,especially at 48h and 72h (compared with MG,P< 0.01 ). Conclusion Aprotinin could alleviate greatly the inflammation and apoptosis following ICH. Inhibiting inflammation response might be one effective way to reduce apoptosis in PHT after ICH.

关 键 词：抑肽酶 脑出血 炎症 凋亡 IL-1Β 

分 类 号：R743.34[医药卫生—神经病学与精神病学]