Interleukin-10 inhibits the expression of nuclear factor kappa B in rats with cerebral ischemia  

作　　者：Zhihua Wu Nan Liu Houwei Du Ronghua Chen An Zheng Huapin Huang 

机构地区：[1]Department of Neurology,the Affiliated Union Hospital of Fujian Medical University,Fuzhou 350001,Fujian Province,China

出　　处：《Neural Regeneration Research》2006年第9期781-784,共4页中国神经再生研究(英文版)

基　　金：grants from Fujian Bureau of Education,No.C0440009;Fujian Bureau of Science and Technology,No.2005K046

摘　　要：BACKGROUND:Plenty of studies have demonstrated that inflammatory reaction is involved in ischemic cerebral damage,and the expression of inflammatory cytokines can be observed at the initial sites of cerebral damage at early period,including interleukin-6,interleukin-8,etc.,which are all the target gene products of nuclear factor kappa B(NF-κB).The process of ischemic damage can be affected by adjusting and controlling NF-κB activity via multi-links.OBJECTIVE:To investigate the inhibitory effect of interleukin-10 on the expression of NF-κB in the ischemic sites of rats with focal cerebral ischemia in rats and its molecular mechanisms.DESIGN:A randomized and controlled animal trial.SETTING:Department of Neurology,the Affiliated Union Hospital of Fujian Medical University.MATERIALS:Thirty-two adult male Sprague-Dawley rats weighing(250±30)g were used.NF-κB p65(RelA)rabbit anti-rat monoclonal primary antibody was the product of Neomarkers Company;Immunohistochemical kit of the SP two-step method was purchased from Beijing Zhongshan Biotechnology Co.,Ltd.METHODS:The experiment was carried out in the Affiliated Union Hospital of Fujian Medical University from August 2005 to April 2006.The rats were randomly assigned into sham-operated group,middle cerebral artery occlusion(MCAO)group,vehicle-treated group and interleukin-10 treated group,8 rats in each group.Focal cerebral ischemia was induced by occlusion of the middle cerebral artery as previously described.Rats in the MCAO group were anesthetized intraperitoneally,thyroid was bluntly dissected.Right common,external and internal carotid arteries were isolated,the trunk of external carotid artery was ligated and freed,an artery clamp was placed at the internal carotid artery,then a'V'shape incision was made at the free section of external carotid artery,filament was inserted for a depth of(18.5±0.5)mm.The rats in the sham-operated group were given the same treatments with the exception of filament insertion.After the successful model establishment for 1 hour,the

分 类 号：R741[医药卫生—神经病学与精神病学]