二烯丙基二硫抑制人胃癌MGC803细胞增殖和对G_2/M期的阻滞作用  

Effect of diallyl disulfide on human gastric carcinoma MGC803 cells proliferation through blockage in G_2/M phase

作　　者：凌杰[1] 金邻杰[1] 

机构地区：[1]台州市第一人民医院,浙江台州318020

出　　处：《现代实用医学》2006年第11期780-781,共2页Modern Practical Medicine

摘　　要：目的研究二烯丙基二硫(DADS)抑制人胃癌MGC803细胞增殖和对G2/M期的阻滞作用。方法采用MTT法测定DADS对MGC803细胞增殖的抑制作用,用流式细胞仪检测DADS对MGC803细胞周期的影响。结果30mg/L、60mg/L、90mg/L的DADS对MGC803细胞的抑制率分别为31.58%±2.20%、53.87%±4.50%、81.58±1.70%,随浓度增高呈上升趋势;对MGC803细胞周期的影响为G0/G1期细胞比率下降,G2/M期逐渐上升。结论DADS对人胃癌MGC803细胞增殖的抑制作用是通过G2/M期阻滞,抑制细胞分裂来完成的。Objective To study the effect of diallyl disulfide(DADS) on human gastric carcinoma MGC803 cells proliferation through blockage in G2/M phase. Methods Inhibition of DADS on MGC803 cells proliferation was measured by MTT assay. The effect of DADS on MGC803 cell cycle was detected by flow cytometry. Results Inhibition ratio of DADS of 30mg/L, 60mg/L and 90mg/L on MGC803 cells were 31.58%±2.20%, 53.87%±4.50%, 81.58%±1.7%,respectively. With the increase of DADS concentration, the rate of G0/G1 phase cells was decreased, while the rate of G2/M phase cells was increased. Condusion DADS can inhibit the proliferation of human gastric carcinoma MGC803 cells, through the blockage of G2/M phase and inhibition of cell division.

关 键 词：胃肿瘤 肿瘤细胞 培养的 细胞分裂 细胞周期 二硫化物类 烯丙化合物 

分 类 号：R735.2[医药卫生—肿瘤]