超低温冻存对人骨髓基质干细胞生物学特性的影响  

Effects of cryopreservation on biological characters of human BMSC

作　　者：郭宗科[1] 章庆国[1] 李静[1] 

机构地区：[1]东南大学附属中大医院整形外科,江苏南京210009

出　　处：《现代医学》2006年第6期376-379,共4页Modern Medical Journal

基　　金：江苏省高新技术研究项目资助(BG2001033);东南大学医学研究基金项目资助(2003YJ25)

摘　　要：目的观察超低温冻存对人骨髓基质干细胞(BMSC)生物学特性的影响。方法从正常献髓者髂骨采集骨髓,分离培养出BMSC,并对其进行成骨诱导培养。将第4代人BMSC经短期超低温冻存、复苏后,通过细胞活性率测定、形态学比较、细胞增殖功能、碱性磷酸酶(ALP)活性检测及体外矿化能力比较,观察超低温冻存对人BMSC生物学特性的影响。结果第4代人BMSC冻存、复苏前后的两组细胞无论在细胞活性率、细胞形态、生长过程、ALP活性等方面均无显著性差异(P>0.05)。结论短期的超低温冻存对人BMSC的生物活性无明显影响。Objective To study the effects of cryopreservation on biological characters of human bone marrow stromal cell(BMSC).Methods BMSC were isolated and cultured from adult bone marrow donor.The cell survival rate,cytomorphology,proliferative abilities,alkaline phosphatase(ALP)activity and the formation of calcified nodules were compared between the 4th generation BMSC and the 4th generation BMSC after short-term cryopreservation.Results There were no differences between two BMSC groups in the cell survival rate,cytomorphology,proliferative abilities and ALP activity(all P>0.05).Conclusion The biological characteristics of BMSC have no changes after short-term cryopreservation.

关 键 词：骨髓基质干细胞 体外培养 超低温冻存 

分 类 号：R-33[医药卫生]