脂氧素A_4对Jurkat T细胞游离钙浓度变化及增殖的影响  

Effects of Lipoxin A_4 on the Changes of Intracellular Free Calcium Concentration and Proliferation in Jurkat T Cells

作　　者：熊维[1] 吴萍[1] 张力[1] 周晓燕[1] 李咏生[1] 叶笃筠[1] 

机构地区：[1]华中科技大学同济医学院基础医学院病理生理学系,武汉430030

出　　处：《华中科技大学学报（医学版）》2006年第6期705-708,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30570726)

摘　　要：目的研究脂氧素A4(LXA4)对Jurkat T细胞内游离钙离子浓度变化及细胞增殖的影响。方法钙离子荧光探针Fluo-3/AM负载细胞后,用激光共聚焦扫描显微镜动态检测活细胞内游离钙离子浓度的变化;CCK-8试剂盒检测细胞增殖情况,并结合流式细胞仪进行细胞周期分析。结果①采用含钙细胞外液时,LXA4降低正常Jurkat T细胞内游离钙离子浓度,而用无钙细胞外液时,细胞内游离钙离子浓度下降不明显;②LXA4呈剂量依赖性抑制钙池操纵钙通道(SOC)激活剂thapsigargin(TG)引起的Jurkat T细胞内游离钙离子浓度的增高,100 nmol/L LXA4也能抑制抗CD3单抗(a-CD3)引起的细胞内钙离子浓度的增高,SOC阻滞剂2-Aminoethoxydiphenylborate(2-APB)能显著抑制TG引起的钙内流;③LXA4呈剂量依赖性抑制a-CD3和抗CD28单抗(a-CD28)诱导的Jurkat T细胞增殖,细胞周期分析发现LXA4阻止Jurkat T细胞由G1期进入S期,降低S期细胞比例、细胞增殖指数(P I)和DNA含量。结论LXA4可能通过抑制Jurkat T细胞钙池操纵的钙通道引起的胞质内游离钙浓度的升高而抑制其增殖。Objective To investigate the effects of lipoxin A_4(LXA_4) on the changes of intracellular free calcium concentration and proliferation in Jurkat T cells.Methods After Jurkat T cells were incubated with Fluo-3/AM,laser confocal scanning microscopy was used to dynamically monitor the changes of intracellular calcium concentration;Cell Counting Kit-8 was used to determine the proliferation of Jurkat T cells,and cell cycle progression was measured by flow cytometry.Results ① LXA_4 decreased the intracellular free calcium concentration of Jurkat T cells in the presence of external Ca^(2+),but the effects were not obvious in the absence of external Ca^(2+);② LXA_4 inhibited the rise of intracellular free calcium concentration of Jurkat T cells induced by thapsigargin(TG),one of the excitomotors of store-operated calcium channel(SOC),in a dose-dependent manner.At the concentration of 100 nmol/L,LXA_4 also inhibited the rise of intracellular free calcium concentration induced by a-CD3;SOC inhibitor 2-APB could suppress the Ca^(2+) entry evoked by TG;③ LXA_4 suppressed the proliferation of Jurkat T cells activated by a-CD3 and a-CD28 antibodies in a dose-dependent manner,as well as the entry of Jurkat T cells from G_1 to S phase and it could decrease the ratio of cells in the S phase,PI values and DNA contents.Conclusion LXA_4 could suppress Jurkat T cells proliferation by inhibiting the rise of intracellular free calcium concentration caused by SOC.

关 键 词：脂氧素A4 JURKAT T细胞 钙池操纵的钙通道 细胞增殖 

分 类 号：R364.5[医药卫生—病理学]