亚硒酸钠对亚砷酸致HepG2细胞DNA损伤的影响  

Effects of Selenium on Arsenic Acid-induced DNA Damage in HepG2 Cells

作　　者：来瑞平[1] 魏巍[1] 余日安[1] 杨成峰[1] 鲁文清[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系教育部环境与健康重点实验室,武汉430030

出　　处：《华中科技大学学报（医学版）》2006年第6期718-720,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30471500)

摘　　要：目的观察硒对砷致HepG2细胞DNA损伤的影响。方法以人肝肿瘤细胞HepG2为靶细胞,分别以亚硒酸钠(2.5、5.0和10.0μmol/L)和亚砷酸(1.56、3.125、6.25、12.5和25.0μmol/L)为受试物处理HepG2细胞,再以亚硒酸钠与亚砷酸联合处理HepG2细胞,应用单细胞凝胶电泳试验检测亚硒酸钠、亚砷酸单独及联合染毒对HepG2细胞DNA的损伤作用。结果与溶剂对照相比,10.0μmol/L亚硒酸钠使HepG2细胞DNA的Olive尾矩显著增加(P<0.05),2.5、5.0μmol/L亚硒酸钠使Olive尾矩有减少的趋势,但差异无显著性意义。6.25、12.5和25.0μmol/L亚砷酸均使HepG2细胞DNA的Olive尾矩显著增加(P<0.01或P<0.05),且呈现剂量依赖关系。25.0μmol/L亚硒酸钠与12.5μmol/L亚砷酸联合作用与12.5μmol/L亚砷酸单独作用相比,Olive尾矩减小,差异有极显著性意义(P<0.01)。5.0μmol/L亚硒酸钠与25.0μmol/L亚砷酸联合作用与25.0μmol/L亚砷酸单独作用相比,Olive尾矩增加,差异有显著性意义(P<0.05)。结论亚硒酸钠对亚砷酸诱导的HepG2细胞DNA损伤的减弱或增强作用与亚硒酸钠和亚砷酸浓度有关。Objective To explore the effects of selenium on arsenic-induced DNA damage in HepG2 cells.Methods As target cells,HepG2 cells were exposed to sodium selenite at the concentrations of 2.5,5.0 and 10.0 μmol/L and to arsenious acid at the concentrations of 1.56,3.125,6.25,12.5 and 25.0 μmol/L respectively.Then the cells were exposed simultaneously to sodium selenite and arsenious acid to explore the effects of co-exposures of sodium selenite and arsenious acid on DNA damage in HepG2 cells.Single cell gel electrophoresis(SCGE) was applied for quantitative analysis of DNA damage.Results Sodium selenite(10.0 μmol/L) could significantly increase DNA Olive tail moment in HepG2 cells in comparison with the solvent control(P<0.05).DNA Olive tail moment was decreased in HepG2 cells treated with sodium selenite of 2.5 and 5.0 μmol/L,but no significant differences were shown as compared to the solvent control.Arsenious acid(6.25,12.5 and 25.0 μmol/L) could significantly increase DNA Olive tail moment in HepG2 cells(P<0.01 or P<0.05) as compared to the solvent control in a dose-dependent manner.Compared with the DNA damage caused by arsenious acid(12.5 μmol/L) exposure alone,DNA Olive tail moment in HepG2 cells was significantly decreased after co-exposure to 2.5 μmol/L of sodium selenite and 12.5 μmol/L of arsenious acid(P<0.01).Conversely DNA Olive tail moment was significantly increased after co-exposure to 5.0 μmol/L of sodium selenite and 25.0 μmol/L of arsenious acid (P<0.05).Conclusion The effect of sodium selenite on arsenious acid-induced DNA damage in HepG2 cells was associated with the concentrations of arsenious acid and sodium selenite.

关 键 词：亚硒酸钠 亚砷酸 DNA损伤 

分 类 号：R730.23[医药卫生—肿瘤]