PEG10基因在不同转移潜能肝癌细胞中表达的定量分析  

Quantitative Analysis of Expression of PEG10 Gene in Liver Cancer Cells with Different Metastatic Potentials

作　　者：张琼[1] 叶达伟[2] 常莹[1] 宋宇虎[1] 谢娜[1] 王晓燕[1] 林菊生[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肝病研究所,武汉430030 [2]华中科技大学同济医学院第二临床学院,2002级武汉430030

出　　处：《华中科技大学学报（医学版）》2006年第6期758-760,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30471983)

摘　　要：目的通过对不同转移潜能的肝癌细胞株中PEG10基因(parternal express gene 10)表达水平的定量分析,探讨其作为肝癌转移基因的可能性。方法提取肝癌细胞株HepG2、MHCC-97L、MHCC-97H的RNA,反转录合成第一链cDNA,对反转录产物进行荧光定量PCR。用管家基因-βactin的cDNA起始浓度作为参照标化PEG10的cDNA起始浓度,对标化结果进行方差分析。结果HepG2、MHCC-97L、MHCC-97H标化后的PEG10 cDNA相对起始浓度分别为(3.15±0.05)×10-3(、5.06±0.03)×10-3(、6.14±0.09)×10-3,各肝癌细胞系之间PEG10表达量差异也存在极显著性意义(P<0.01)。结论PEG10基因表达水平与肝癌细胞的转移潜能呈正相关,可以推测PEG10基因可能是促使肝癌转移的癌基因。Objective To explore the possibility of PEG10 as a metastasis oncogene by quantitatively analyzing the expression of PEG10 in liver cancer cell strains with different metastatic potentials.Methods Total RNA was extracted from liver cancer cell strains,HepG2,MHCC-97L and MHCC-97H.The first strand cDNA was synthesized by reverse transcription,which was then used as the template to perform fluorescent quantitative polymerase chain reaction(FQ-PCR).The quantity of the expression of PEG10 gene was normalized by dividing with that of the housekeeping gene,β-actin for each sample.One-way analysis of variance(ANOVA) was performed for the normalized values.Results The normalized initial cDNA concentrations of PEG10 in HepG2,MHCC-97L and MHCC-97H were(3.15±0.05)×10^(-3),(5.06±0.03)×10^(-3),(6.14±0.09)×10^(-3),respectively.There was significant difference in the expression level in liver cancer cell strains with different metastatic potentials.Conclusion The expression levels of PEG10 gene in liver cancer cells were positively correlated with their different metastatic potentials,speculating that PEG10 may be a metastasis suppressor gene for liver cancer.

关 键 词：肝癌 肿瘤转移 荧光定量PCR PEG10基因 

分 类 号：R735.7[医药卫生—肿瘤]