体外超声激活血卟啉单甲醚抗C6胶质瘤细胞的研究  

Bioeffect Induced by HMME Activated by Ultrasound on C6 Glioma Cells

作　　者：岳武[1] 宋大勇[1] 孔令秀[2] 赵钊[1] 孟祥喜[3] 张旭[3] 王殿洪[3] 王策[1] 

机构地区：[1]哈尔滨医科大学第四临床医学院神经外科,哈尔滨市150001 [2]哈尔滨吉秀高新技术股份有限公司 [3]哈尔滨医科大学第一临床医学院神经外科

出　　处：《中国激光医学杂志》2006年第5期278-282,共5页Chinese Journal of Laser Medicine & Surgery

摘　　要：目的研究超声激活血卟啉单甲醚(HMME),对C6胶质瘤细胞的生物效应,为声动力疗法(sonodynamic therapy,SDT)治疗脑胶质瘤奠定实验基础。方法实验分为SDT组(超声+HMME组)、超声组、HMME组及仅加入肿瘤细胞的对照组。HMME终浓度为10μg/ml,SDT组、超声组进行超声照射(频率：1．0mHz,声强度：0．5W/cm^2,时间：120s)处理。采用噻唑蓝比色分析法(MTT法)观察SDT处理后6、12、24、48和72h的抑瘤率；流式细胞学检测SDT处理后6h C6细胞凋亡率及细胞周期百分比。结果SDT组抑瘤率显著增高；超声组也有一定的抑瘤率；HMME组抑瘤率与对照组无明显差别。SDT后凋亡率升高,G_0/G_1期、G_2/M期百分比降低,S期百分比升高、凋亡增殖比(APR)升高。结论SDT能显著杀伤C6胶质瘤细胞,并诱导其凋亡,使细胞阻滞于S期→G_2期。Objective To study the bioeffect induced by HMME activated by ultrasound on C6 glioma cells,and lay the experimental foundation for glioma treatment by sonodynamic therapy. Methods The glioma cells were divided into four groups:including SDT group(ultrasound with HMME);ultrasonic group;HMME group and control group.The SDT group and ultrasonic group were treated by ultrasound(frequency:1.0 mHz,sound intensity: 0.5W/cm^2,time:120s)with HMME(concentration:10μg/ml).The inhibition rate of C6 glioma cells at different times(6,12,24,48 and 72 h)after SDT treatment was investigated by MTT assay.The apoptotic rate and cell cycle of C6 glioma ceils were detected by flow cytometry(FCM). Results The inhibition rate of C6 glioma cells after SDT significantly increased;ultrasonic group also had some effect;the inhibition rate of hematoporphyrin monomethyl ether(HMME)group had no significant difference compared with control group.Treated with SDT,the apoptotic rate increased and the percentage of G_0/G_1 and G_2/M decreased,the percentage of S increased and the apoptosis rate increased. Conclusions SDT can significantly kill C6 glioma cells in vitro,induce apoptosis of C6 cells and arrest cell cycle at S/G_2.

关 键 词：声动力疗法 血卟啉单甲醚 凋亡 

分 类 号：R739.4[医药卫生—肿瘤]