NOTCH-1信号通路对人脑胶质瘤干细胞增殖和分化作用的研究  

NOTCH-1 signaling pathway regulates proliferation and differentiation of human brain glioma stem cells

作　　者：于春泳[1] 尹昌林[1] 吕胜青[1] 杨辉[1] 

机构地区：[1]中国人民解放军第三军医大学新桥医院神经外科,重庆400037

出　　处：《中国微侵袭神经外科杂志》2008年第6期268-272,共5页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：重庆市自然科学基金资助项目(编号:CSTC;2006BB5092;CSTC;2006BB5109)

摘　　要：目的研究NOTCH-1信号通路对人脑胶质瘤干细胞增殖和分化的作用及意义。方法应用免疫磁珠法从人脑胶质瘤组织标本和胶质瘤细胞株中提取、分选胶质瘤干细胞,进行体外培养;应用免疫组织化学、免疫荧光双标法方法和RT-qPCR法分别检测其增殖、分化过程中NOTCH-1信号通路中关键基因和蛋白的表达情况。结果人脑胶质瘤组织中存在胶质干细胞。NOTCH-1在CD133、MAP2、GFAP和MBP阳性细胞中均可表达,在CD133阳性细胞中最显著。胶质瘤干细胞增殖过程均能检测到较强的NOTCH-1信号通路关键基因NOTCH-1、CBF-1和HES-1的mRNA表达,在胶质瘤干细胞分化时,表达逐渐减弱。结论NOTCH-1基因在人脑胶质瘤中高表达;NOTCH-1信号通路参与了胶质瘤干细胞增殖、分化过程,为人脑胶质瘤治疗的新靶点提供了依据。Objective To investigate effect and significance of NOTCH-1 signaling pathway on proliferation and differentiation of human brain glioma stem cells (BGSCs). Methods Specimens of glioma were obtained from patients undergoing microsurgical tumor resection. BGSCs were isolated from these tumor specimens and sorted by magnetic activated cell sorting, and then cultured and proliferated ex vivo. The expressions of the key gene and protein in the NOTCH-1 signaling pathway in the course of proliferation and differe...

关 键 词：神经胶质瘤 干细胞 信号传导 

分 类 号：R739.4[医药卫生—肿瘤]