原发性肝癌外周血白蛋白基因片段扩增的临床意义  

Clinic significance of the albumin gene fragment amplified in peripheral blood with hepatocellular carcinoma

作　　者：朱云松[1] 杨大明[2] 姚登福[2] 孟宪镛[2] 李玉明[1] 钱俊波[1] 王亚明[1] 许利华[1] 

机构地区：[1]南通大学第二附属医院消化科 [2]南通大学附属医院,南通226001

出　　处：《南通大学学报（医学版）》2008年第4期261-263,共3页Journal of Nantong University(Medical sciences)

摘　　要：目的:探讨外周血白蛋白(Alb)基因在肝癌早期诊断和鉴别诊断中的临床价值。方法:以巢式聚合酶链反应(RT-PCR)扩增外周血中Alb的基因片段分析在原发性肝癌(HCC)、慢性肝病和非肝病患者外周血中血白蛋白基因表达。结果:67例肝癌患者外周血中Alb基因的检出率为59.7%,明显高于肝硬化、慢性肝炎和肝外肿瘤组患者(P<0.01),但与急性肝炎组无明显差别(P>0.05);在Ⅰ、Ⅱ和Ⅲ期肝癌中,Alb基因片段阳性率分别为33.3%、36.8%和76.9%;肝内有无转移的肝癌组间存在明显差异(P<0.05),尤其在伴有肝外远处转移的肝癌中,Alb基因全数阳性(100.0%)。结论:分析外周血中Alb基因有助于肝癌的诊断、肝癌远处转移判别的监测。Objective:To explore clinical values of peripheral blood albumin gene in early diagnosis and differential diagno- sis of hepatocellular carcinoma (HCC).Methods:The Alb gene was amplified by nested reverse transcription polymerase chain reaction (RT-PCR) in patients with hepatocellular carcinoma,chronic liver or non-liver disease.Results:The positive rate of peripheral blood Alb gene fragments from 67 patients with HCC was 59.7%.It was significantly higher than that from the pa- tients with liver cirrhosis (...

关 键 词：原发性肝癌 Alb基因 RT-巢式PCR 

分 类 号：R735.7[医药卫生—肿瘤]