RT-PCR检测穹窿海马伞切割后大鼠海马内Lhx8 mRNA的表达变化  

Expression change of Lhx8 mRNA in rats hippocampus after fimbria fornix transected by using RT-PCR

作　　者：秦建兵[1] 金国华[1] 蒋媛媛[1] 王磊[1] 朱蕙霞[1] 田美玲[1] 张新化[1] 

机构地区：[1]南通大学医学院人体解剖学教研室神经生物学研究所江苏省神经再生重点实验室,南通226001

出　　处：《南通大学学报（医学版）》2008年第4期253-255,共3页Journal of Nantong University(Medical sciences)

基　　金：江苏省高校自然科学基金(KJB180111);江苏省高等学校研究生创新计划项目(200584)

摘　　要：目的:探讨切割穹隆海马伞大鼠海马与正常海马内Lhx8 mRNA表达的差异。方法:36只SD大鼠随机分成6组,每组6只。1组为正常对照组,其余5组分别为切割穹窿海马伞后3、7、14、21和28 d组。取各组大鼠的海马组织,提取总RNA,采用半定量RT-PCR法分析穹隆海马伞切割后海马内Lhx8 mRNA的表达变化。结果:海马内Lhx8 mRNA的表达量在切割后第3 d开始升高,7 d时达到最高水平,以后逐渐下降,于28 d时恢复至正常水平。结论:切割穹窿海马伞后海马中Lhx8 mRNA表达的增高可能与神经干细胞向胆碱能神经元分化的再生机制有关。Objective:To observe the difference of Lhx8 mRNA expression in hippocampi between the fimbria fornix tran- sected rats and normal ones.Methods:36 SD rats were randomly divided into 6 groups,6 rats in each group.Group one was the control group,and the others were 3rd,7th,14th,21st and 28th day group after fimbria fomix transected.Then hip- pocampi were isolated and total RNA was extracted.Semi-quantitative reverse transcriptase-polymerase chain reaction (RT- PCR) analysis was used to detect the expressions o...

关 键 词：穹窿海马伞切割 海马 Lhx8 逆转录-聚合酶链反应 大鼠 

分 类 号：R[医药卫生]