盐酸多奈哌齐对血管性痴呆小鼠海马ERK1及ERK2表达变化的影响  

The Effect of Donepezil Hydrochloride on the Changes about ERK1 and ERK2 at the Hippocampus of Mice with Vascular Dementia

作　　者：吕佩源[1] 胡亚卓[1] 郭宗成[1] 胡爱祥[1] 李玲[1] 梁翠萍[1] 何春年[2] 翟金萍[2] 

机构地区：[1]河北省人民医院神经内科,050051 [2]河北省人民医院病理科

出　　处：《脑与神经疾病杂志》2008年第2期84-86,110,共4页Journal of Brain and Nervous Diseases

基　　金：国家人事部留学人员科技活动择优资助项目(2001-119-1)

摘　　要：目的:探讨盐酸多奈哌齐对血管性痴呆(VD)小鼠海马中细胞外信号调节激酶(ERK1、ERK2)表达变化的影响。方法:采用双侧颈总动脉反复缺血-再灌注法制备小鼠VD模型。将小鼠随机分为假手术组、模型组及药物组,药物组给予盐酸多奈哌齐灌胃治疗。术后第29、30天,经跳台试验和水迷宫试验对各组小鼠进行行为学成绩测试,用免疫组化方法观察各组小鼠海马CA1区ERK1、ERK2的表达变化。结果:盐酸多奈哌齐明显改善了VD小鼠学习、记忆成绩(p<0.05)。模型组小鼠海马CA1区ERK1、ERK2表达较假手术组及治疗组减少,均有显著性差异(p<0.05)。结论:ERK1、ERK2的表达减少可能参与了血管性痴呆的发病机制,盐酸多奈哌齐通过增加乙酰胆碱含量,后者作用于毒蕈碱乙酰胆碱受体(M受体)激活ERK,有助于改善学习和记忆功能。Objective:To observe the effect of donepezil hydrochloride on the changes about extracellular signal-regulated kinase(ERK1 and ERK2)at the hippocampus of mice with vascular dementia(VD).Methods:Kunming mice were divided into sham group,model group and treatment group with donepezil hydrochloride randomly.The models of VD mice were established through three times ischemia-reperfusion of bilateral common carotid arteries.The mice from drug group were treated with donepezil hydrochloride.The behavioral scores ...

关 键 词：血管性痴呆 小鼠 细胞外信号调节激酶 免疫组化 海马 

分 类 号：R749.16[医药卫生—神经病学与精神病学]