Apelin激活大鼠主动脉一氧化氮系统的研究  

Apelin Activates Nitric Oxide Pathway in Isolated Aorta in Rat

作　　者：贾月霞[1] 杨靖辉[2] 潘春水[2] 李桂忠[1] 耿彬[2] 赵晶[2] 唐朝枢[2] 齐永芬[2] 

机构地区：[1]宁夏医学院病理生理学教研室,银川750004 [2]北京大学医学部生理与病理生理学系,北京100083

出　　处：《宁夏医学院学报》2005年第6期429-431,共3页Journal of Ningxia Medical College

基　　金：国家重大基础研究项目(973)(No:G2000056905);国家自然科学基金(No:30470693);宁夏自然科学基金资助项目(No:NZ0542)

摘　　要：目的研究Apelin对大鼠主动脉L-精氨酸转运、一氧化氮合酶(NOS)活性和NO生成的影响.方法体外血管薄片孵育,测定孵育液中亚硝酸盐(NO2-)含量、L-精氨酸转运及血管NOS活性.结果大鼠主动脉孵育3h后,Apelin(10-9～10-7mol/L)呈浓度依赖地刺激血管NO2-生成增加(33%、46%和69%,与对照组比较,均P＜0.01);血管总NOS活性呈浓度依赖性(total nitric oxide sythase,tNOS)增加,分别较对照组增加0.68、2.36和2.5倍(均P＜0.01),且以内皮型NOS(endothelialNOS,eNOS)活性升高(均为P＜0.01)为主,较对照组分别增加2、4.6和5.5倍,而诱导型NOS(inducible NOS,iNOS)活性并无明显改变.结论Apelin通过激活血管组织NOS/NO系统,增加血管L-精氨酸转运与NO生成,引起血管的扩张.Objective To investigated the activation of apelin in the transport of L-arginine and nitric oxide synthase(NOS)/nitric oxide(NO) pathway in isolated incubated aorta in rat.Methods The aortic slices were incubated in vitro.The medium nitrite content,vascular NOS activities and L-arginine transport were measured.Results After incubation of aorta for 3 hour,nitrite production increased markedly compared with control(33%、46% and 69%,all P<0.01) in apelin dose dependent manner.The total NOS activities elevated significantly(0.68,2.36,2.5times) compared with control in apelin dose dependent manner.The endothelial NOS(eNOS) activities enhanced mainly(2,4.6,5.5times) compared with control(all P<0.01).However,the inducible NOS activities did not change(P>0.05).Conclusion These results suggest that apelin activate(NOS/NO) and link to the transport of L-arginine and the increase of NO contents and vasodilatation in aorta.

关 键 词：APELIN L-精氨酸 一氧化氮合酶 一氧化氮 

分 类 号：R363[医药卫生—病理学]