假单胞菌O-2-2利用油脂废水生产鼠李糖脂研究  

Study on rhamnolipid production from refined vegetable oil wastewater by Pseudomonas sp.O-2-2

作　　者：梁生康[1] 王修林[1] 单宝田[1] 汪卫东[2] 

机构地区：[1]中国海洋大学化学化工学院,山东青岛266003 [2]胜利油田有限公司采油工艺研究院,山东东营257000

出　　处：《现代化工》2005年第z1期192-196,共5页Modern Chemical Industry

基　　金："十五"科技攻关项目(2002BA904B06);青岛市科技发展计划项目(2004-2-JZ-108)资助

摘　　要：通过摇瓶培养试验,研究了铜绿假单胞菌O-2-2以植物油精炼废水为原料生产鼠李糖脂生物表面活性剂的适宜条件.结果表明以NaNO3为氮源,碳氮比为15,培养基初始pH值为7.5,32℃,250 r/min培养84 h,鼠李糖脂产量最高,达2.14g/L.液-质联用分析结果表明所提取的糖脂由11种同系物组成,都含有1～2个的鼠李糖和1～2个含β-羟基的碳链长度为8～12的饱和或不饱和脂肪酸.该糖脂生物表面活性剂的临界胶束浓度为69.5 mg/L,可将水的表面张力降至29.2 mN/m,并具有良好的耐温耐盐性.The optimal conditions of flask culture for rhamnolipid biosurfactant production from refined vegetable oil wastewater were studied through Pseudomonas aeruginosa strain O-2-2.The maximal rhamnolipid concentration of 2.14 g/L can be reached when the strain is incubated for 84 h at such conditions:NaNO_3 as nitrogen source,15 of C/N ratio,7.5 of initial pH value,at 32℃ and 250 r/min of shaking rate.Totally 11 rhamnolipid homologs,being formed by one or two rhamnose molecules linked to one or two β-hydroxy fatty acids of saturated or unsaturated alkyl chain between C_8 and C_(12),were isolated and identified from the glycolipid mixture by HPLC-ESI-MS.The critical micelle concentration of the rhamnolipid biosurfactant was 69.5 mg/L.It can lower the water surface tension to 29.2 mN/m and keep its surface activity at high temperature or in high-salinity solution.

关 键 词：生物表面活性剂 鼠李糖脂 铜绿假单胞菌O-2-2 植物油精炼废水 

分 类 号：TQ423[化学工程] TQ920.1[轻工技术与工程—发酵工程]