浮游球衣菌单株培养形态学特征的研究  

Morphology features cultivating Sphaerotilus natans by single strain research

作　　者：叶兰[1] 

机构地区：[1]安徽省环境监测中心站,合肥230061

出　　处：《南京大学学报（自然科学版）》2008年第4期441-446,共6页Journal of Nanjing University（Natural Science）

基　　金：国家自然科学基金(49672102)

摘　　要：浮游球衣菌是污水好氧生化处理中的知名菌种,对污水中有机物和重金属有很强的降解和去除作用.本文经分离纯化后,对单个菌株进行连续培养.该菌株在极低营养环境(A培养基)中呈现树枝状向四周辐射的菌落,在普通琼脂平板,呈光滑园形隆起的白色菌落.对数期的细胞10~12 min分裂一次;单胞菌体和短丝体阶段均强烈摆动.培养4 d后,菌丝达十几个到数十个细胞长时,丝体相互缠结成团,丝体中细胞大量逸出胶鞘.电镜片显示,菌体细胞两端均有纤维状鞭毛并相互连接成丝体,胞外有宽厚的半透明胶鞘.该菌具有在低营养环境中强力吸取营养的特性,以及快速繁殖和强烈的活动性,与其在污水处理中的卓越效果可能有关联.长丝体缠结成团时,丝体中细胞大量逸出,仅含胶质鞘团块的比重必然减轻,这可能是污泥膨胀的主要原因.在培养后期溶解氧(DO)有明显下降,也可能与污泥澎胀有因果关系.Sphaerotilus natans is a well-known strain in the environmental microbiology.In this paper the very low culture concentration is adopted for its isolation and purification(one-tenth of the commonly-used culture medium).The ends of some fine fibrous mycelium formed on the culture medium are picked and readmitted with inoculation needle so that the efficiency of isolation and purification become higher.The strain after purification is continuously diluted and cultured until monocelled strain is obtained.Based...

关 键 词：浮游球衣菌 单株培养 形态学特征 电镜分析 

分 类 号：X172[环境科学与工程—环境科学]