多环境因素全面正交作用对铜绿微囊藻生长的效应研究  

The overall orthogonal design study of multifactor interaction on the growth of Microcystis aeruginosa in the presence of adnascent Pseudomonas sp.

作　　者：袁丽娜[1] 宋炜[1] 肖琳[1] 蒋丽娟[1] 杨柳燕[1] 

机构地区：[1]污染控制与资源化研究国家重点实验室南京大学环境学院,南京210093

出　　处：《南京大学学报（自然科学版）》2008年第4期408-414,共7页Journal of Nanjing University（Natural Science）

基　　金：国家自然科学基金(40371102;30770414);江苏省太湖专项项目(BK2007738);国家重点基础研究发展规划(973)项目(2002CB412307)

摘　　要：应用全面正交设计试验方案,研究与附生细菌共生时,温度、pH值、氮磷比三因素的不同水平组合与铜绿微囊藻生长的动态关系.结果表明,在环境温度25℃、氮磷比40∶1、初始pH为11时,铜绿微囊藻的最终生长量最大;温度25℃、氮磷比16∶1、初始pH为8.2时次之,微囊藻细胞的比增长速率相差约1.9%.统计分析结果显示,温度和初始pH值是影响铜绿微囊藻生长状况的显著因素.低温(10℃)显著抑制微囊藻的增长,低温时铜绿微囊藻对其他环境因子(pH值和氮磷比)的变化基本无响应;低pH值和较低氮磷比的组合也能明显抑制微囊藻生长,藻细胞不增长甚至处于负增长状态,此时温度对铜绿微囊藻比增长速率和最终生物量的影响程度也相应降低.Microcystis aeruginosa is a famous bloom-forming cyanobacteria,which often occurs in eutrophic lakes,such as Tai Lake.Mathematical models were established for the relations between the biomass of Microcystis aeruginosa in the presence of Pseudomonas sp.and different treatments of N/P ratio,pH and tempreture in this paper.Three levels of each factor were set.After 16 days combination cultivation,the results were as follows: the maximum biomass of M.aeruginosa occurred when N/P ratio was 40∶1,pH was 11 and t...

关 键 词：温度 PH值 氮磷比 铜绿微囊藻 正交试验 

分 类 号：X524[环境科学与工程—环境工程]