survivin基因启动子驱动的肿瘤特异性腺病毒载体的构建  

Construction of a tumor specific adenoviral vector piloted by survivin promoter

作　　者：白万胜[1] 成诗银[1] 王军利[1] 卞卡[1] 张惠中[2] 刘丽[2] 

机构地区：[1]第四军医大学唐都医院耳鼻咽喉科,西安710038 [2]第四军医大学唐都医院临床实验科,西安710038

出　　处：《陕西医学杂志》2008年第8期959-961,共3页Shaanxi Medical Journal

基　　金：国家自然科学基金资助项目(No:30572024)

摘　　要：目的:构建肿瘤特异性启动子驱动的腺病毒载体,检测survivin基因启动子在喉癌细胞中的特异表达活性。方法:PCR扩增survivin启动子,构建分别携带survivin启动子和CMV启动子的真核表达载体pCMV-EGFP和pSurp-EGFP,体外连接法制备重组腺病毒Ad-SP-EGFP和Ad-CMV-EGFP,后转染Hep-2细胞及血管内皮细胞ECV304,荧光显微镜下观察EGFP表达。结果:成功构建survivin基因启动子的腺病毒载体;Ad-SP-EGFP转染后荧光镜下见Hep-2细胞发出较强的绿色荧光,而ECV304细胞无表达。结论:成功制备肿瘤特异性survivin启动子驱动的腺病毒,为进一步开发肿瘤的特异性基因治疗奠定了实验基础。Objective:To construct adenoviral vector piloted by tumor specific promoter and to detect the specific expression of survivin gene promoter in laryngocarcinoma cells.Method:The survivin gene promoter was generated by PCR to produce the eukaryotic expression vector pCMV-EGFP and pSurp-EGFP carrying with CMV promoter or survivin promoter.The original recombinant recombinant viruses Ad-SP-EGFP and Ad-CMV-EGFP were harvested by ligation in vitro.The two adenovirus were transfected into Hep-2 cell and vessel end...

关 键 词：喉肿瘤 基因疗法 @腺病毒载体 @survivin基因 

分 类 号：R739.65[医药卫生—肿瘤]