含Arg-Gly-Asp肽与仿生PLGA-(ASP-PEG)材料结合的实验研究  

Arg-Gly-Asp-containing Peptide Combining with the Biomimetic And Modified PLGA-(ASP-PEG)

作　　者：宋玉林[1] 郑启新[1] 郭晓东[1] 郝杰[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院骨科,武汉430022 [2]首都医科大学附属北京友谊医院骨科,北京100000

出　　处：《生物医学工程学杂志》2008年第4期860-863,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30170270,30200063)

摘　　要：设计合成含Arg-Gly-Asp[Arg(R)-精氨酸,Gly(G)-甘氨酸,Asp(D)-天冬氨酸]的非病毒基因载体的肽(K16-GRGDSPC)(K-赖氨酸,S-丝氨酸,P-脯氨酸,C-半胱氨酸),用其作材料聚(丙交酯-co-乙交酯)-(天冬氨酸-聚乙二醇)交替预聚物[PLGA-(ASP-PEG)]的表面修饰。合成K16-GRGDSPC,将PLGA-(ASP-PEG)制成A、B、C三块薄片。薄片A、B与交联剂反应,反应后的薄片A再与肽反应,薄片C作为对照。质谱仪(MS)、高效液相色谱分析仪(HPLC)检测肽的分子量及纯度;x线光电子能谱法(XPS)检测材料表面硫元素;分光光度仪检测残液未反应肽含量。HPLC检测肽的纯度为94.13%,MS检测肽分子量为2741.26。XPS检测改性薄片A中硫元素结合能为164eV,碳、硫元素的含量比值(C/S)为99.746∶0.1014;反应后薄片B中硫元素结合能为162eV与164eV,C/S为99.574∶0.4255;薄片C中无硫元素。残液管OD值为0.069,薄片A表面接枝肽密度为0.04mg/mm2。提示通过交联剂可将所合成的肽K16-GRGDSPC结合到仿生材料PLGA-(ASP-PEG)表面。Arg-Gly-Asp-(RGD) containing peptide characterized as the non-viral gene vector was synthesized to modify the surface of PLGA-(ASP-PEG). The Peptide (K16-GRGDSPC)was synthesized. PLGA-(ASP-PEG) was executed into chips A, B and C. Chip C was regarded as control. Chips A and B reacted with the cross-linker, then Chip A reacted with peptide. Mass spectrometry(MS) and high performance liquid chromatography(HPLC) detected the molecular weight and the purity of peptide. Sulphur in the surface of materials was det...

关 键 词：ARG-GLY-ASP 仿生 表面修饰 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]