佛波酯对食管癌EC9706细胞NDRG_1表达及细胞增殖影响的研究  

Effects of 12-tetradecanoyl-phorbol-13-acetate on Expression of NDRG_1 mRNA and Proliferation of Human Esophageal Carcinoma EC9706 Cells

作　　者：尹惠卿[1] 贺付成[1] 张岚[2] 张云汉[2] 

机构地区：[1]郑州大学第一附属医院检验科,郑州450052 [2]河南省肿瘤病理重点实验室

出　　处：《陕西医学杂志》2008年第7期776-778,共3页Shaanxi Medical Journal

基　　金：国家自然科学基金资助项目(No30470779)

摘　　要：目的:探讨12-氧-十四烷酰佛波醇-13-乙酸酯(TPA)作用于食管癌细胞EC9706后,对NDRG1mRNA表达、细胞增殖、细胞周期的影响。方法:采用50nmol/L的TPA作用于EC9706细胞,MTT法检测细胞增殖情况,流式细胞仪检测细胞周期的变化,原位杂交法检测NDRG1mRNA水平。结果:TPA能上调NDRG1mRNA水平,抑制EC9706细胞增殖,细胞周期发生G1→S期阻滞。结论:TPA可能通过上调NDRG1mRNA的表达而抑制EC9706增殖,促进其分化。Objective:To study the expression of NDRG1 mRNA,proliferation and cell cycle distribution of human esophageal carcinoma EC9706 cells after being treated with TPA.Methods:EC9706 cells were treated with 50nmol/L TPA,detecting the cell proliferation by MTT method and observing cell cycle distribution by Flow-cytometry,in situ hybridization technique to detect the NDRG1 mRNA level.Results:TPA could inhibit the proliferation of EC9706 cells,bring about G1 phase arrest and up regulate the expression level of NDRG...

关 键 词：食管肿瘤/免疫学 佛波醇酯类/分析 细胞分裂 @EC9706细胞 @NDRG1基因 细胞周期 基因表达 

分 类 号：R735.1[医药卫生—肿瘤]