MIF在大鼠重症急性胰腺炎相关肺损伤中的作用  

Effects of MIF on severe acute pancreatitis-associated lung injury in rats

作　　者：王烜[1] 张瑞[1] 李丽[1] 邓明明[1] 

机构地区：[1]泸州医学院附属医院消化内科,泸州646000

出　　处：《陕西医学杂志》2008年第7期803-805,共3页Shaanxi Medical Journal

基　　金：四川省泸州市科技局资助项目[泸市科(2004)62号]

摘　　要：目的:探讨MIF在大鼠重症急性胰腺炎相关肺损伤发病机制中的作用。方法:30只雌性SD大鼠随机分为正常对照组、胰腺炎2、4、8、12h组。采用5%牛磺胆酸钠逆行胰胆管注射的方法,复制大鼠重症急性胰腺炎相关肺损伤模型。分别对胰腺损伤、肺损伤程度进行病理评分;测定肺组织湿/干质量比、胰腺组织湿重、血清淀粉酶;用ELISA法检测血清MIF含量;用半定量RT-PCR法检测肺组织中MIFmRNA水平。结果:造模后各组肺损伤评分、胰腺损伤评分、肺组织湿/干质量比、胰腺组织湿重及血清淀粉酶均逐渐升高;血清及肺组织中MIF均较正常组升高。结论:大鼠重症急性胰腺炎相关肺损伤发病过程中,血清及肺组织MIF表达明显增加,提示MIF在重症急性胰腺炎相关肺损伤中可能起重要作用。Object:To study the effect of MIF on mechanism of rats severe acute pancreatitis associated lung injury.Method:Thirty female Sprague-Dawley(SD)rats were divided into normal group、2 hours group、 4 hours group、8 hours group and 12 hours group.1ml/kg body weight of 5% sodium taurocholate was retrograde injected into the biliopancreatic duct of the rats to induce SAP.Pathological evaluations of pancreas and lung were made.Blood were obtained for detecting plasma amylase.Pancreatic wet weight,lung wet/dry weight...

关 键 词：胰腺炎/并发症 肺疾病/病因学 巨噬细胞游走抑制因子/分析 大鼠 

分 类 号：R576[医药卫生—消化系统]