多花黄精挥发油GC-MS分析及其生物活性研究  

Study on Constituents and Biological Activity of Volatile Oil From Tubers of Polygonatum cyrtonema Hua

作　　者：余红 张小平[1] 邓明强 叶红翠 蒋继宏[2] 

机构地区：[1]安徽省重要生物资源保护与利用研究重点实验室 [2]江苏省药用植物生物技术重点实验室,江苏徐州221116

出　　处：《中国实验方剂学杂志》2008年第5期4-6,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：教育部科学技术研究重点项目(206066);安徽省自然科学基金项目资助项目(050430501);高等学校博士点专项科研基金(20060370001)

摘　　要：目的:分析多花黄精根状茎挥发油成分及检测其抑菌和抗肿瘤生物活性。方法:采用水蒸汽蒸馏法(SD)提取挥发油,用气相色谱-质谱(GC-MS)联用技术分析多花黄精根状茎精油的成分,生物活性检测采用杯碟法和Alamar blue法。结果:共鉴定出16个化合物,占总挥发油的95.97%,该挥发油对大肠杆菌、金黄色葡萄球菌、红酵母均有较强的抑制能力;对人肺癌细胞(NCI-H460)有显著的抑制作用,当浓度达到100μg.mL-1,抑制率达到98.08%。结论:实验结果表明多花黄精挥发油成分具有较强的生物活性,为进一步研究其药理作用提供了科学依据。Objective:To analyse the constituents and determine the biological activity of volatile oil from the tubers of Polygonatum cyrtonema.Methods:The volatile oil was obtained by steam distillation.And its chemical constituents were analyzed by GC-MS.Biological activity of the volatile oil was screened with microbiological method and Alamar Blue method.Results:16 compounds were identified,making up 95.97% of the total contents;The volatile oil could inhibit the growth of Escherichia coli、Staphylococcus aureus an...

关 键 词：多花黄精 挥发油 气相色谱-质谱联用 生物活性 

分 类 号：R284.1[医药卫生—中药学]