mRNA转染树突细胞协同CIK细胞体内抗肿瘤作用的研究  

Co-antitumor effect of CIK cells and dendritic cells transfected with mRNA in vivo

作　　者：谢云青[1] 林晓为[1] 郑秋红[1] 汪相如[1] 龚福生[1] 王晓盈[1] 

机构地区：[1]福建医科大学教学医院福建省肿瘤医院肿瘤研究所肿瘤分子生物学研究室,福建福州350014

出　　处：《中华肿瘤防治杂志》2008年第12期890-893,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：福建省自然科学基金(F00019)

摘　　要：目的:研究人肺腺癌细胞系SPC-A1 mRNA基因转染的树突状细胞(dendritic cells,DCs)和细胞因子诱导活化杀伤细胞(cytokine induced killer,CIK)体内协同抗瘤活性。方法:按常规方法分离健康人外周血单个核细胞,并分别诱导培养为DC和CIK细胞。将人肺腺癌细胞mRNA转染DC细胞,并将转染后成熟DC与CIK细胞混合培养,流式细胞仪检测混合培养前后的CIK细胞表型变化,并通过建立人肺腺癌细胞移植模型研究其体内的协同抗肿瘤活性。结果:混合培养后的CIK细胞表面CD3+CD8+及CD3+CD56+表达显著升高,P<0.01;共孵育后的mRNA-DC-CIK细胞组与其余各组相比,体内肿瘤抑制效率明显增大。结论:利用该方法来负载DC瘤苗具有可行性,为临床上解决DC瘤苗因肿瘤抗原的来源困难问题及提高CIK细胞的特异性抗肿瘤活性提供实验依据,为肺癌的治疗开辟一种新的有效的免疫治疗策略。OBJECTIVE: To study the co-antitumor effect of cytokine induced killer(CIK) cells and dendritic cells(DCs) transfected with human adenocarcinoma of lung cell line SPC-A1 mRNA.METHODS:The peripheral blood mononuclear cells were isolated and induced into DC and CIK cells.The health human peripheral blood DC cells were transfected with human adenocarcinoma of lung cell line SPC-A1 mRNA,and then co-cultured with CIKcells,and the surface markers on CIK cells were tested with flow cytometry.The anti-tumor activit...

关 键 词：肺肿瘤/免疫学 树突细胞 杀伤细胞 RNA 信使 细胞毒性 免疫 

分 类 号：R73-36[医药卫生—肿瘤]