放射诱导的六株细胞系细胞周期和细胞凋亡变化的观察  

Properties of apoptosis and cell cycle in six radiation-induced cell lines

作　　者：刘沙[1] 董广璐[1] 刘伟[2] 金茜[2] 张淑云[2] 郑淑香[1] 邢丽娜[1] 

机构地区：[1]哈尔滨医科大学附属第二医院肿瘤放射治疗科,黑龙江哈尔滨150086 [2]哈尔滨医科大学附属第二医院肿科研实验中心,黑龙江哈尔滨150086

出　　处：《中华肿瘤防治杂志》2008年第13期988-990,995,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：黑龙江省科技计划攻关项目基金(GC03C606-4)

摘　　要：目的:探讨放射诱导的6株细胞系细胞周期和细胞凋亡的变化特点。方法:正常肝细胞系HL-7702,肝癌细胞系HepG2和SMMC-7721,肺小细胞癌HCI-H460,肺腺癌A549和宫颈癌细胞系Hela常规培养48h后接受4GyX射线照射;收获受照前(0h)和受照后6、12、24、36和48h的细胞,采用流式细胞术(FCM)检测各细胞系细胞凋亡和细胞周期。结果:在4GyX线照射前,SMMC-7721和HCI-H460细胞凋亡率明显高于其他4株细胞系,两者差异有统计学意义(t=20.98,P<0.005);在照射后12h,与照射前相比6株细胞系细胞凋亡率均有显著增加(t=5.27,P<0.05),SMMC-7721、HCI-H460和A549同时伴有S期和G2～M期细胞比率的降低;在照射后36hHCI-H460出现第2个细胞凋亡峰,伴有极低比例的S期和G2～M期细胞;HepG2在照射后12h、HL-7702和Hela在照射后24h均有明显的G2/M期阻滞。结论:4GyX线诱导的细胞凋亡主要发生在射线照射后12～36h,6株细胞系可能均发生了"有丝分裂前凋亡";每株细胞还呈现了不同的细胞凋亡和细胞周期变化特点,具有组织细胞特异性。OBJECTIVE:To analysis the apoptosis and cell cycle of 6 radiation-induced cell lines,including HL-7702,HepG2,SMMC-7721,HCI-H460,A549 and Hela.METHODS:After being cultured for 48 hours the 6 cells were irradiated at 4 Gy X-radiation.The cell cycle and apoptosis of cells after being irradiated for 0,6,12,24,36 and 48 hours were detected by FCM.RESULTS:Before being irradiated at 4 Gy X-radiation,the apoptosis rates of SMMC-7721 and HCI-H460 were significantly higher than those of the other 4 cell lines(t=20.98...

关 键 词：肿瘤/放射疗法 细胞凋亡/辐射效应 肿瘤细胞 培养的 细胞周期 

分 类 号：R730.55[医药卫生—肿瘤]