基于HPV16肿瘤基因治疗载体的构建与鉴定  

Construction and identification of tumor gene therapy vector based on human papilloma virus type 16

作　　者：杨曌[1] 王惠明[1] 

机构地区：[1]第三军医大学基础部全军免疫研究所,重庆400038

出　　处：《中华肿瘤防治杂志》2008年第13期965-967,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30400215)

摘　　要：目的:构建基于人乳头状瘤病毒16型(human papilloma virus type-16,HPV16)的肿瘤基因治疗载体,并鉴定其对Hela肿瘤细胞的转染活性。方法:利用昆虫杆状病毒表达系统表达病毒蛋白,在体外变性与复性过程中用病毒蛋白包装绿色荧光表达质粒EGFP形成治疗载体。透射电镜观察治疗载体的结构,并转染Hela肿瘤细胞,荧光显微镜和流式细胞仪检测其转染效率。结果:透射电镜观察证实病毒蛋白可自我组装成病毒样颗粒(virus-like particle,VLP)的结构,在转染Hela肿瘤细胞后,荧光显微镜和流式细胞仪成功检测到荧光的表达。结论:基于HPV16的新型肿瘤基因治疗载体能成功转染Hela肿瘤细胞,为肿瘤的基因治疗策略提供了理想的工具。OBJECTIVE:To constructa tumor gene therapy vector based on human papilloma virus type 16 and examine its transfection activity in vitro.METHODS:The virus protein was expressed by the Bac-to-Bac expression system,and EGFP and virus were formed into pseudovirus in the course of degeneration and regeneration in vitro.The construction was identified by a transmission electron microscope and the transfection activity was detected by fluoroscope and FCM.RESULTS:The pseudovirus could selfassemble into VLP,and the ...

关 键 词：乳头状瘤病毒 人 基因疗法 遗传载体 

分 类 号：R73-36[医药卫生—肿瘤]