己烯雌酚对成年仓鼠附睾组织形态的影响  

Effects of diethylstilbestrol on morphology of adult hamster epididymis

作　　者：杨筱珍[1] 陈耀星[1] 王子旭[1] 

机构地区：[1]中国农业大学动物医学院

出　　处：《中国兽医学报》2008年第6期694-696,701,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(39500108,30270960);上海市博士启动资金资助项目(05-212);上海市重点学科建设项目(Y1101)

摘　　要：探讨环境内分泌干扰物已烯雌酚(DES)对成年动物附睾组织形态的影响,将DES皮下注射成年仓鼠(剂量为每天1mg/kg)连续1周后,用光镜和电镜观察附睾组织形态的变化,并用脱氧核糖核酸末端转移酶介导的dUTP缺口末端标记法(TUNEL)检测附睾管上皮细胞和精子的凋亡情况。结果,DES处理后附睾头和附睾尾萎缩,附睾管变细。附睾管上皮细胞形态出现明显的改变,附睾上皮细胞和精子均出现大量凋亡,附睾管管腔内充满发育异常的生精细胞,少见长形精子。结果表明,DES能诱发成年动物附睾形态的改变,这可能是造成成年动物生精障碍的重要原因。To explore the effects of diethylstilbestrol(DES)as environment endocrine disruptors on epididymis morphology of adult hamster,the DES(1mg /kg body weight)was treated daily by subcutaneous injection for one week.Morphological changes on adult hamster epididymis were observed by light and transmission electron microscope.Apoptotic epithelial cells of epididymal tubules and spermatogenetic cells were detected by TUNEL staining.The results showed:In parallel,both caput and caudal epididymis occurred atrophy,an...

关 键 词：己烯雌酚 仓鼠 附睾 凋亡 

分 类 号：R361[医药卫生—病理学]