茯苓多糖对受照射白血病K562细胞N-乙酰氨基半乳糖转移酶-9和自由基等的影响  

Effects of Pachyman Polysaccharides on ppGaINAc-T9 and Free Radical in the Radiated K562 Cells

作　　者：刘可人[1] 杨雪枫[1] 吴士良[1] 范雁[1] 徐爱华[1] 

机构地区：[1]苏州大学医学院生化与分子生物学教研室苏州大学生化工程研究所,苏州215007

出　　处：《中国中西医结合杂志》2005年第S1期94-96,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国防基础科研计划基金(No.2003044);苏州大学医学发展基金(No.2003007)资助项目

摘　　要：目的用化学方法提取茯苓多糖,并观察其对肿瘤细胞的作用。方法利用乙酸沉淀法、DEAE-纤维素层析法提取并分离茯苓多糖,并运用RT-PCR方法和流式细胞技术,检测其对受照射后肿瘤细胞的多肽：N-乙酰氨基半乳糖转移酶-9(ppGalNAc-T9)mRNA表达、自由基活力以及细胞周期的影响。结果茯苓多糖可使经γ射线照射后的K562细胞中的自由基增多、ppFalNAc-T9表达增高、G_1期受阻明显。结论茯苓多糖对肿瘤细胞中自由基具有一定的清除作用,同时还可以增加ppGalNAc-T9在mRNA水平的表达。Objective To observe the effect of pachyman polysaccharides(PPS)on tumor cells.Methods The PPS was extracted and isolated by Acetic acid sedimentation hrethod.The effect of PPS was observed by chemi- cal methods,and the expression of ppGalNAc-T9,the free radical activity and cell cycle of the exposed tumor cells were determined by RT-PCR method and flow cytometry.Results PPS could increase the free radical,enhance the expression of ppGalNAc-T9 and obviously obstruct the G_1 phase in the K562 cell afterγ-irr...

关 键 词：茯苓多糖 肿瘤 RT-PCR 多肽 N-乙酰氨基半乳糖转移酶 自由基 细胞周期 

分 类 号：R285[医药卫生—中药学]