粒细胞集落刺激因子对大鼠颈动脉PCI后内膜修复和增生的影响  

Effects of granulocyte-colony stimulating factor on reendothelialization and intima hyperplasia in rat carotidartery injuried by ballon catheter

作　　者：崔连群[1] 王晓军[1] 刘继东[1] 王敏[1] 盖玉生[1] 李峰[1] 

机构地区：[1]山东济南山东省立医院内科,250021

出　　处：《介入放射学杂志》2005年第S1期90-92,共3页Journal of Interventional Radiology

摘　　要：目的　探讨重组人粒细胞集落刺激因子(rhG CSF)对大鼠颈总动脉球囊损伤后再内皮化和内膜增生的影响。方法　5 6只Wistar大鼠随机分为2组。观察组2 8只:皮下注射rhG CSF30 μg·kg- 1 ·d- 1 。7d后行左颈总动脉球囊导管拉伤。术后1h、3、5、7、14d用扫描电镜、伊文氏蓝染色、HE染色、免疫组织化学方法,观察血管再内皮化、新生内膜增厚及细胞增殖情况。对照组2 8只:用生理盐水代替rhG CSF ,其余同观察组。结果　发现观察组的内膜再生面积(An)和An At(内膜损伤总面积)比值明显高于对照组(P <0 .0 1) ;内膜厚度(Ti)、Ti Tm(中膜厚度)比值及PCNA阳性细胞率均明显低于对照组(P<0 .0 1)。结论　干细胞动员剂rhG CSF显著促进球囊损伤后血管内皮化、抑制SMC和内皮细胞过度增生。Objective To study the effects of mobilization agent of bone marrow stem cells, exogenous recombinant human granulocyte-colony stimulating factor (rhG-CSF), on the repair process of reendothelialization and neointima hyperplasy of rat carotid arteries injured with a balloon catheter. Methods A model of vascular restenosis was established on carotid arteries injured by a balloon catheter in 56 male Wistar rats randomized classified as rhG-CSF group and Contrast group.The animals were injected daily wit...

关 键 词：粒细胞集落刺激因子 内皮祖细胞 大鼠 颈动脉 

分 类 号：R363[医药卫生—病理学]