银耳多糖对实验性衰老小鼠的保护作用  

Protective effect of tremella polysaccharides on experimental aging mouse model

作　　者：李燕[1] 蔡东联[1] 胡同杰[1] 陈小莉[1] 

机构地区：[1]第二军医大学长海医院营养科,上海200433

出　　处：《第二军医大学学报》2004年第10期1104-1107,共4页Academic Journal of Second Military Medical University

基　　金：上海市科委发展基金 ( 0 2 41190 48)

摘　　要：目的 :观察银耳多糖对 D-半乳糖所致衰老小鼠的作用。方法 :4 0只雄性 ICR小鼠 ,随机分为 5组 ,正常对照组腹腔注射生理盐水 ,其余 4组每天腹腔注射 D-半乳糖 12 0 m g/ kg,连续 6 0 d,建立亚急性衰老模型。同时在这 4组中 ,衰老对照组灌胃生理盐水 ,其余 3组每天分别灌胃 80 0、4 0 0和 2 0 0 mg/ kg银耳多糖。实验结束后 ,比色法检测各组小鼠心、脑中氧化性指标丙二醛 (MDA)与脂褐质 (L P)的含量 ,抗氧化酶 SOD与 GSH- Px的活性以及小鼠皮肤中羟脯氨酸含量 ,免疫组化法检测肾脏中细胞周期调节因子 P2 1的表达情况 ,MTT法检测 Con A诱导的小鼠脾淋巴细胞增殖转化。 结果 :各银耳多糖干预组 SOD及 GSH- Px高于衰老对照组 ,而 MDA及 L P低于衰老对照组 ,并均有显著性差异 (P<0 .0 5 ) ;银耳多糖能明显促进 Con A诱导的小鼠淋巴细胞增殖转化 ;各银耳多糖干预组肾脏 P2 1的表达量比衰老对照组低 (P<0 .0 5 ) ,而皮肤羟脯氨酸含量比衰老对照组高 (P<0 .0 5 )。 结论 :银耳多糖对 D-半乳糖所致的衰老有一定的改善作用。Objective:To observe the protective effect of tremella polysaccharides (TP) on mouse aging model induced by D-galactose. Methods: Forty male ICR mice were divided into 5 groups (Group A: treated with 800 mg/kg TP; Group B: treated by 400 mg/kg TP; Group C: treated by 200 mg/kg TP; Group D: aging control; Group E: normal control). Except for Group E,other groups were injected with D-galactose [120 mg/(kg·d)] for 60 d to establish subacute aging model. Group A,B and C were treated with 800,400 and 200 mg/kg TP respectively via intragastric (i.g.) administration. Sixty days later,the spleen,heart, kidney and brain were collected to determine MDA, LP, SOD, GSH-Px and contents of hydroxyproline by colorimetry. Expression of P21 in the kidney were detected by immunohischemical technique. Proliferation and transformation of spleen lymphocytes induced by ConA were determined by MTT method. Results: Antioxidants SOD and GSH-Px activity of TP treated groups were higher than aging control group (P<0.05); MDA and LP of TP treated groups were also lower than those of aging control group (P<0.05). TP obviously promoted the lymphocytes to proliferate induced by ConA in vitro. Furthermore, in TP treated groups, the kidney expression of cell cycle regulator P21 were lower and hydroxyproline contents in the skin were higher than those in Group D (P<0.05). Conclusion: It is suggested that TP can prevent aging of mice induced by D-galactose.

关 键 词：银耳多糖 D-半乳糖 衰老 小鼠 

分 类 号：R339.38[医药卫生—人体生理学]