MALDI-TOF-TOF鉴定IPG IEF分离的蛋白质  

Identification of IPG IEF Separated Proteins by MALDI - TOF - TOF

作　　者：蒙根[1] 李卫华[1] 王鸿丽[1] 谷苗[1] 刘锋[1] 王杰[1] 魏开华[1] 

机构地区：[1]军事医学科学院国家生物医学分析中心,北京100850

出　　处：《分析测试学报》2004年第z1期74-75,共2页Journal of Instrumental Analysis

基　　金：国家863高技术发展项目(2001AA233041)

摘　　要：　　K562细胞是一株分化差、恶性程度高的人白血病细胞.研究表明,在一些分化诱导剂的作用下,细胞可以向红细胞系、粒细胞系和巨K562核细胞系方向分化成熟,并表现出相应血细胞类型的成熟标志.用一维固相pH梯度等电聚焦电泳(LPG IEF)分离K562细胞总蛋白质,其每一条带常包含多个蛋白质,难以用肽指纹谱技术来鉴定.……Proteins from human erythroleukemia K562 were separated with IPG IEF, 27 bands were excised and then digested in gel. MALDI - TOF - TOF Ultraflex was used for protein identification under automatic MS - MS mode. 45 proteins, such as heterogeneous nuclear ribonucleoprotein, prohibitin, chaperonin, were identified with more than 3 MS - MS data for each protein. The highest score of 213 matched with 7 peptides.

关 键 词：TOF-TOF Protein identification Tandem MS PROTEOMICS Human erythroleukemia 

分 类 号：O657-55[理学—分析化学]