尼古丁对体外培养鼠磨牙牙胚发育的影响  

Effect of nicotine on mouse molar germ development in vitro

作　　者：王小竞[1] 文玲英[1] 杨富生[1] 吴礼安[1] 刘宏[2] 关口浩[3] 药师寺仁[3] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032 [2]第二军医大学长海医院,上海210000 [3]日本东京齿科大学小儿齿科学讲座

出　　处：《牙体牙髓牙周病学杂志》2004年第3期130-133,共4页Chinese Journal of Conservative Dentistry

摘　　要：目的 :探讨尼古丁对体外鼠磨牙牙胚发育的影响。方法 :采用RPMI- 16 4 0半固态培养基 ,在培养基中加入不同浓度的尼古丁 ,将 15d胎龄的胎鼠的下颌第一磨牙牙胚分别置于各浓度组RPMI- 16 4 0半固态培养基表面培养 4、8d ,每 4 8h更换培养基一次。结果 :实验组与对照组相比较 ,主要表现在实验组发育中的牙胚体积缩小 ,成牙本质样细胞的分化程度不足、数量和速度均低于对照组 ,前期牙本质样基质形成减少或不能形成 ,并且呈时间、浓度依赖性。结论 :尼古丁对体外培养胎鼠牙胚的形态、细胞的增殖和分化有抑制作用。AIM:To study the effect of nicotine on mouse molar dental germs development in vitro .MTTHODS:With RPMI-1640 semisolid medium, nicotine of different concentration was added into, first mandibalar molar germs from 15-day old mouse embryos were seperated and cultured on semisolid medium for 4 and 8 days. Every 48 h the medium changed once.RESULTS:It showed that the volume of developing tooth germs was decreased,number and rapidity of odontoblasts in expreimental group were lower than those in control group.The predentin-like matrix decreased or could not form. It showed dose-and-time-dependent.CONCLUSION:Nicotine inhibited the morphologic size of mouse tooth germs.proliferation and differentiation of cells in vitro .

关 键 词：尼古丁 鼠磨牙牙胚 发育 

分 类 号：R780.2[医药卫生—口腔医学]