气道上皮细胞趋化活性测定方法的研究  

A Study on Chemotaxis of Airway Epithelial Cells

作　　者：管茶香[1] 张长青[1] 秦晓群[1] 罗自强[1] 刘惠君[1] 

机构地区：[1]中南大学湘雅医学院生理学系,中国湖南长沙410078

出　　处：《实用预防医学》2004年第4期644-645,共2页Practical Preventive Medicine

基　　金：国家自然科学基金资助项目 (NO 3980 0 0 53)

摘　　要：目的　探讨气道上皮细胞趋化活性的测定方法 ,建立气道上皮损伤修复的评价指标。　方法　以不同浓度的胰岛素作为趋化因子 ,测定气道上皮细胞的趋化活性。同时观察选用不同孵育时间时气道上皮细胞趋化活性的变化。　结果　在一定浓度范围内 ,气道上皮细胞的趋化活性随胰岛素浓度的升高而增加 ;孵育时间以 6h最佳。　结论　本文建立的气道上皮细胞趋化活性的测定方法稳定可靠。Objective To investigate the method of chemotactic migration of bronchial epithelial cells( BECs). Methods Determine chemotaxis of BECs by using insulin as chemotactic factor in different concentration and cultured time. Result A concentration dependent response between insulin and the chemotaxis of BECs was found. The optimal chemotactic migration of BECs were cultured within 6 h. Conclustion The method for determing chemotaxis of BECs was stable and dependable.

关 键 词：气道上皮细胞 趋化活性 测定方法 

分 类 号：R332.2[医药卫生—人体生理学]