玻璃化法对离子注入拟南芥幼苗过程中的冻害防护效应  

The Protective Effect of Vitrification on Freeze Injury of Arabidopsis thaliana Seedling During Ion Beam Implantation

作　　者：谷运红[1,2] 余增亮[3] 秦广雍[1] 霍裕平[1] 

机构地区：[1]郑州大学河南省离子束生物工程重点实验室 [2]中国科学院等离子体物理研究所离子束生物工程学重点实验室,合肥230031 [3]中国科学院等离子体物理研究所离子束生物工程学重点实验室

出　　处：《植物生理学通讯》2003年第5期425-428,共4页Plant Physiology Communications

基　　金：国家自然科学基金重大项目课题 ( 19890 3 0 0 );国家"十五"科技攻关项目 ( 2 0 0 1BA3 0 2B 0 3 )

摘　　要：离子注入过程中活体材料往往由于真空造成的冻害而不能存活。文章以拟南芥幼苗为对象 ,尝试了玻璃化法对注入过程中冻害的防护效应。结果显示 ,玻璃化处理的 2d龄幼苗经液氮冻融处理后存活率最高 ,达 80 %以上。 2d龄幼苗经玻璃化处理后进行离子注入 ,在一定剂量范围内成活率在 90 %以上 ;而未经玻璃化处理的幼苗离子注入后均不能成活。玻璃化后注入的幼苗成活率随注入剂量的增加而呈下降趋势 ,但受注入的离子能量的影响不大。显示玻璃化法对离子注入过程中活体材料遭受的冻害有较好的防护效果 ,在应用上有一定的意义。In vitro material is always difficult to survive because of freeze injury during ion beam implantation. The protective effect of vitrification on freeze injury of Arabidopsis thaliana during Ion Beam Implantation was studied. The results showed that 2 d old seedling had the highest survival rate (>80%) when they were treated by vitrification and liquid N 2. The survival rate of 2 d old seedling implanted by ion beam was higher than 90% within a suitable dose range. But the survival rate of control was 0. The survival rate of 2 d old seedling implanted by ion beam decreased with the increasing of dose. But energy of ions had little influence on seedling survival rate. It was indicated that vitrification had the protective effect on freeze injury of Arabidopsis thaliana during ion beam implantation.

关 键 词：玻璃化 离子注入 拟南芥幼苗 冻害 

分 类 号：S33[农业科学—作物遗传育种]