奶水牛β-酪蛋白启动子的克隆及其序列分析  

CLONING AND SEQUENCING ANALYSIS OF BUFFALO β- CASEIN PROMOTER

作　　者：董克家[1] 

机构地区：[1]海南医学院转基因实验室,海口571101

出　　处：《海南医学院学报》2003年第6期321-322,共2页Journal of Hainan Medical University

基　　金：教育部高等学校骨干教师计划(2000-65);海南省百项农业新技术基金(1998-149)资助。

摘　　要：目的:克隆水牛乳腺β-酪蛋白启动子。方法:分离提取水牛乳腺组织DNA,PCR扩增目的片段,T-A克隆,核酸自动测序。结果:所克隆的DNA片段包含CAAT、TATA信号区,孕酮调节区等,100%同源于黑白花奶牛β酪蛋白启动子序列。结论:所克隆DNA片段是奶水牛β-酪蛋白基因上游调控序列。Objective To clone the promoter of buffalo β-casein gene. Method The buffalo DNA was extracted rom a hybrided-buffalo mammary gland.The promoter of buffalo β-casein gene was amplified by PCR which was cloned into the pGEM-T Easy vector. Results The sequencing analysis showed the promoter included CAAT and TTA signal ?progesterone regulatory region as well as octamer motif which was 100 percent of homologous to that of the milk cow. Conclusion Our results suggested. The DNA fragment is the buffalo β-casein upstream regulatory sequence.

关 键 词：水牛 β酪蛋白 启动子 

分 类 号：R34[医药卫生—基础医学]