2-丙烯酰胺基-2-甲基丙磺酸/丙烯酸共聚物合成及与牛血清蛋白络合研究  

Synthesis of copolyelectrolyte and complexation with bovine serum albumen

作　　者：梁世飘 李峰[2] 王颖[2] 陈晓欢[2] 

机构地区：[1]西安交通大学生命科学与技术学院,陕西西安710049 [2]西安交通大学环境与化学工程学院,陕西西安710049

出　　处：《现代化工》2003年第z1期191-192,共2页Modern Chemical Industry

摘　　要：在水溶液中pH值7.5的条件下将2-丙烯酰胺基-2-甲基丙磺酸(AMPS)与丙烯酸(AM)共聚,得到带有一定负电荷密度的水溶性聚合物。研究该聚合物和生物活性大分子牛血清蛋白的络合作用,采用紫外吸收光谱测定了该聚合物与牛血清蛋白的络合比值为1:1.48,并初步探讨其络合机理。A copolymer of 2-acrylamido-2-methylpr-opanesulfonate (AMPS) and acrylamide (AM) was prepared by solu- tion polymerization at pH 7.5. The copolymer was water soluble, with a fixed quantity of negative charge. The complexation be- tween poly(AMPS-AM) and bovine serum albumen(BSA) was studied by using ultraviolet absorbing spectral analysis and it's found that the complex ratio was 1: 1. 48. The complexation mechanism was also discussed elementarily.

关 键 词：2-丙烯酰胺基-2-甲基丙磺酸 丙烯酸 牛血清蛋白 生物分离 

分 类 号：O631[理学—高分子化学]