重组大肠杆菌VG1(pTU14)合成3-羟基丁酸酯-3-羟戊酸酯共聚物的研究  

Research on PHBV fermentation with recombined E.coli VG1(pTU14)

作　　者：周航[1] 于慧敏[1] 沈忠耀[1] 

机构地区：[1]清华大学化工系生物化工研究所,北京100084

出　　处：《现代化工》2003年第z1期198-201,208,共5页Modern Chemical Industry

基　　金：国家"九五"攻关项目(96-03-03-02);国家自然科学基金(29834103);国家自然科学基金(29876021)

摘　　要：摇瓶培养结果表明,重组大肠杆菌 E.coli VG1(pTU14)可以在培养基中加入丙酸的环境下合成聚(3-羟基丁酸-3-羟基成酸酯)(PHBV),且丙酸的初浓度和加入时间是影响细菌生长、PHBV积累和产品中HV分率的关键因素。在摇瓶补料分批培养中,通过监测细胞生长、PHBV积累和丙酸的消耗过程,优化并确定了丙酸的补料策略。E.coli VG1(pTU14)经过48 h的摇瓶补料分批培养,菌体干重、PHBV浓度、PHBV含量和HV摩尔分率,分别达到16.6 g/L、13.1 g/L、78.7％和7.2％。PHBV相对于葡萄糖的得率、HV相对于丙酸的得率和PHBV的产率分别为0.327 g/g、0.343 g/g和0.273 g/L/h。The flask culture revealed that E. coli VG1(pTU14) can synthesize PHBV [Poly(3-hydroxybutyrate-co-3-hy- droxyvalerate)] in the culture medium containing propionic. The time of adding propionic and its concentration had significant effects on cell growth, PHBV synthesis, and HV fraction in the copolymer. In the fed-batch culture, the dynamic process of cell growth, PHBV accumulation and propionic consumption were studied, and finally an optimal feeding process of propionic was put forward. Afer 48h of fed-batch culture, the DCW (dry cell weight), the PHBV concentration, the PHBV content and the HV fraction in PHBV reached 16. 6 g/L, 13. 1 g/L, 78. 7% and 7. 2 mol%, respectively. The yield of PHBV to glucose, the yield of HV to propionic and PHBV productivity reached 0. 327 g/g,0. 343 g/g and 0. 273 g/L/h, respectively.

关 键 词：重组大肠杆菌 PHBV 丙酸 

分 类 号：TQ929[轻工技术与工程—发酵工程]