番荔枝内酯单体89-2实验治疗KBv200及KB细胞移植瘤的作用(英文)  

Experimental chemotherapy against xenografts derived from multidrug resistant KBv200 cells and parental drug-sensitive KB cells in nude mice by annonaceous acetogenin 89-2

作　　者：符立梧[1] 何丽容[1] 梁永钜[1] 陈黎明[1] 熊慧瑜[1] 杨小平[1] 潘启超[1] 

机构地区：[1]中山大学肿瘤防治中心,广东广州510060

出　　处：《药学学报》2003年第8期565-570,共6页Acta Pharmaceutica Sinica

基　　金：NationalNaturalScienceFoundationofChina(39800182),MedicalScienceTechniqueFoundationofGuangdongProvince(A19982 2 2 )

摘　　要：目的　番荔枝内酯单体 89 2是自阿蒂莫耶番荔枝植物分离获得。本研究旨在探讨 89 2对MDR肿瘤的实验治疗作用。方法　以MTT法测定细胞毒 ;KBv2 0 0及KB细胞裸鼠移植瘤模型研究 89 2对MDR肿瘤的实验治疗作用 ;以Fura 2 AM法测定P 糖蛋白 (P gp)功能。结果　 89 2对KBv2 0 0及KB细胞的IC50 分别为 4 8 7和 6 4 6nmol·L- 1(P >0 0 5 )。 89 2对KB及KBv2 0 0细胞裸鼠移植瘤具有相似的剂量依赖性抑制作用 ,而毒性可以耐受。 89 2剂量依赖性地增加KBv2 0 0细胞的Fura 2积累。结论　 89 2体内外均抑制KB和KBv2 0 0细胞生长 。Aim Annonaceous acetogenin 89 2 was obtained from atemoya plant. To investigate the effect of 89 2 on experimental chemotherapy against xenografts derived from multidrug resistant KBv200 cells and parental drug sensitive KB cells. Methods Cytotoxicity was determined by tetrazolium (MTT) assay. The models of KB and KBv200 xenografts in nude mice were established to investigate the effect of 89 2 on experimental chemotherapy against cancer in vivo . Mechanistic experiments were conducted to examine t...

关 键 词：番荔枝内酯 多药抗药性 P-糖蛋白 抗肿瘤作用 FURA-2 

分 类 号：R73-36[医药卫生—肿瘤]