β-淀粉样肽_(25-35)致记忆障碍大鼠中枢双孔钾通道亚型mRNA表达的改变  

mRNA expression alteration of two-pore potassium channels in the brain of β-amyloid peptide_(25-35)-induced memory impaired rats

作　　者：潘雅萍[1] 徐向华[1] 王晓良[1] 

机构地区：[1]中国医学科学院,中国协和医科大学药物研究所,北京100050

出　　处：《药学学报》2003年第10期721-724,共4页Acta Pharmaceutica Sinica

基　　金：国家"973计划"项目资助 (G19980 5110 6 )

摘　　要：目的　研究聚集态 β 淀粉样肽2 5 3 5(β AP2 5 3 5)致记忆障碍大鼠模型中枢双孔钾通道亚型mRNA表达的变化。方法　Morris水迷宫实验检验大鼠空间学习记忆能力 ,用RT PCR方法检测大脑皮层和海马中 3种双孔钾通道TREK 1 ,TREK 2和TRAAKmRNA的表达。结果　在Morris水迷宫实验中 ,β AP2 5 3 5注射组大鼠d 1 ,d 2和d 4的潜伏期均比对照组显著延长。β AP2 5 3 5注射组大鼠大脑海马中TREK 1 ,TREK 2和TRAAKmRNA的表达比对照组明显升高 ;而在皮层中的表达变化不显著。结论　侧脑室注射聚集态 β AP2 5 3Aim To study mRNA expression alteration of two pore potassium channels in the brain of β amyloid peptide 25 35 (β AP 25 35 ) induced memory impaired rats. Methods Memory impairments induced in rats by single icv injection of β AP 25 35 (2 mmol·L -1 ) 5 μL were assessed in the Morris water maze test. The mRNA expression levels of three two pore potassium channels TREK 1, TREK 2 and TRAAK were detected in rat cerebral cortex and hippocampus by reverse transcription polymera...

关 键 词：Β-淀粉样肽 双孔钾通道 逆转录聚合酶链式反应 

分 类 号：R749.1[医药卫生—神经病学与精神病学]