甲型副伤寒沙门氏菌体抗原抗血清的简易提纯  

Preparation and simple purification of multivalent antiserum of somatic antigen of Salmonella paratyphi A

作　　者：袁耀武[1] 张伟[1] 李英军[1] 马雯[1] 

机构地区：[1]河北农业大学食品科技学院生物工程系,河北保定071001

出　　处：《免疫学杂志》2003年第S1期45-47,共3页Immunological Journal

摘　　要：目的 单克隆抗体以其结合抗原的专一性用于血清学检验是一种必然趋势,而多克隆机体则以其简易的制备方式在目前仍得到广泛的应用,但多克隆抗体的提纯仍有许多繁琐之处,使其优势受到影响。本试验以甲型副伤寒沙门氏菌体抗原多克隆抗体的制备及提纯为例,提出可行的更简易的提纯方法。方法 利用产G蛋白链球菌,以一种较为简易的特异性结合和解离的方法来纯化甲型副伤寒沙门氏菌体抗原免疫血清。结果 得到了特异性IgG抗体。结论 利用这种纯化方法在实验室简易可行。Objective Using of monoclonal antibody is a certain direction in serological examination because of its specificity combined with antigen, so polyclonal antibody is in use widely at present for its simple way of preparation . However, there is a lot of trouble in the course of purification of polyclonal antibody, this reduces its advantage. In this experiment , we took the example of preparation and purification of polyclonal antibody of somatic antigen of Salmonella paratyphi A and found out a simpler feas...

关 键 词：单克隆抗体 多克隆抗体 菌体抗原 

分 类 号：R392-33[医药卫生—免疫学]