4℃平衡时间对PCK细胞低温保存的影响(英文)  

Effect of the equilibrium time at 4℃ on the cryopreservation of PCK cells

作　　者：陈少鹏[1] 杨先乐[1] 瞿小英[1] 黄艳平[1] 芮行知 

机构地区：[1]上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海200090

出　　处：《上海水产大学学报》2003年第S1期1-5,共5页Journal of Shanghai Fisheries University

基　　金：The open-oriented subject of Chang Jiang Fisheries Research Institution. E2001-8

摘　　要：主要阐述了在以二甲亚砜为抗冻剂,不同的4℃平衡时间对PCK细胞低温保存的影响。把第24代PCK细胞悬浮于含10％二甲亚砜(DMSO)的冻存保护液,置于4℃冰箱,按不同的时间间隔取出细胞,重新培养,用来研究不同的平衡时间对细胞的贴壁率及生长的影响。将19代PCK细胞,按40min、1h、2h三种不同的4℃平衡时间进行-700℃冻存,再以较低的细胞密度(2×10~4个/mL)复苏细胞,用以研究不同的平衡时间对细胞冻存的复苏率及形态学的影响。结果表明,较长时间放置在4℃,DMSO的毒副作用明显,不利于细胞的生长。同直接传代的细胞相比,放置24h以上明显影响细胞的贴壁率及单层形成时间。在PCK细胞的-70℃保存中,4℃下平衡40min,DMSO不能对细胞形成充分的保护,细胞碎片较多;平衡2h以上,DMSO有影响了细胞对低温伤害的抵御能力,同样不利于细胞的冻存;平衡1h,细胞复苏及生长情况最佳。This paper mainly described the effect of the different equilibrium time on cryopreservation of PCK cells when dimethylsulphoxide (DMSO) was used as the cryoprotectant. PCK cells at passage 24 were suspended in M199 with 20% newborn bovine serum(NBS) and 10% DMSO, then they were placed at 4t. The cells were taken out by different time intervals. After centrifugation and the discard of cryopreservative solution, the cells were cultured again. This was used to study the effect of different equilibrium time at...

关 键 词：PCK细胞系 细胞培养 低温保存 抗冻剂 复苏率 平衡时间 

分 类 号：Q25[生物学—细胞生物学]