高温蛋白酶产生菌株YMTC1049的筛选与鉴定  

Screening and characterization of thermophilic strains which producing thermophilic protease

作　　者：祝伟[1] 彭谦[1] 郭春雷[1] 张东华 刘杨 

机构地区：[1]云南大学云南省微生物研究所教育部微生物资源开放研究重点实验室,云南昆明650091

出　　处：《云南大学学报（自然科学版）》2003年第S1期22-27,共6页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(NO39960003);云南省工业微生物发酵工程重点实验室开放基金资助项目.

摘　　要：从腾冲热海温泉样品中分离的53株蛋白酶产生菌,经初筛和复筛,获得1株高温蛋白酶活力为46.80U/mL,编号为YMTC1049的菌株.根据形态特征、生理生化特征和16SrDNA序列分析比较,将YMTC1049菌株鉴定为泥土芽孢杆菌属的菌株(Geobacillussp.).After primary screening and secondary screening,thermophilic bacterium strain YMTC?1049 which highly produced thermophilic protease was isolated from the samples from Tengchong.Strain YMTC1049 was most similar in morphological and physiological charateristers to Geobacillus genus.Phylogenetic analysis based on 16SrDNA sequences showed that strain YMTC?1049 is most closely related to Geobacillus stearothermophilus.Based on the data of phenotypic properties and 16?SrDNA sequence analysis,strain YMTC?1049 ...

关 键 词：高温蛋白酶 酶活力 泥土芽孢杆菌属(Geobacillus) 鉴定 

分 类 号：Q93-33[生物学—微生物学]