多种反义寡脱氧核苷酸联合逆转人肝癌多药耐药性的实验研究  

Antisense Phosphorothioate Oligodeoxynucleotides in Reversal of Multidrug Resistance of Human Hepatic Cancer Cell Line

作　　者：刘景丰[1] 洪伟[1] 

机构地区：[1]福建医科大学附属第一医院肝脏外科中心,福建福州350005

出　　处：《中西医结合肝病杂志》2003年第S1期19-22,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:探讨反义寡脱氧核苷酸(ASPODNS)对人肝癌多药耐药基因(MDR)的逆转作用。方法:ASPONs作用于肝癌MDR细胞模型后,用MTT法检测其逆转作用,用逆转录PCR和流式细胞术检测其对4种MDR基因表达的影响。结果:ASPODNs抑制MDR基因的表达存在时间-效应和剂量-效应关系,对单基因MDR细胞耐药性的逆转率为90.26％～100％,对多重MDR细胞耐药性逆转率为70.09％～100％。结论:多种ASPODNs联合逆转MDR具有可行性并能取得明显效果。Objective: To study the role of antisense phosphorothioate oligodeoxynucleotides (ASPODNs) in reversal of multidrug resistance (MDR) of human hepatic cancer cell line. Methods: The reverse rate of ASPODNs to MDR was analysed by MTT assay and the effect of ASPODNs on MDR gene expression at mRNA by RT-PCR and at proteins by flow cytometry. Results: ASPODNs almost completely inhibited related MDR gene s expression. This inhibition was time-effective and dose-ef-fective. The reverse rates of ASPODNs to MDR indu...

关 键 词：寡核苷酸类 反义 癌 肝细胞 抗药性 多药 

分 类 号：R735.7[医药卫生—肿瘤]