shRNA对胃癌细胞株SGC-7901 VEGF-C表达的抑制作用  

Inhibitory Effect of shRNA on Expression of the VEGF-C in SGC-7901 Cells

作　　者：达明绪[1] 罗婷[2] 赵紫罡[3] 王捷[1] 

机构地区：[1]中山大学附属第二医院普外科,广东广州510120 [2]四川大学华西医院,四川成都610041 [3]包头市肿瘤医院外二科,内蒙古包头014000

出　　处：《华西医学》2008年第5期1083-1086,共4页West China Medical Journal

基　　金：广东省自然科学基金No.07301381;中国博士后科学基金No.20060400777

摘　　要：目的:筛选出有效干扰胃癌SGC-7901细胞株血管内皮生长因子-C(vascular endothelial growth factor C,VEGF-C)的短发卡状RNA(short hairpin RNA,shRNA)。方法:设计2个shRNA片段,VEGFC-1505与VEG-FC-1417,体外转染VEGF-C阳性表达的胃癌细胞株SGC-7901,采用RT-PCR检测VEGF-C mRNA表达水平的变化,western-blot检测VEGF-C蛋白水平变化。结果:VEGFC-1505可以显著抑制VEGF-C基因表达,对其mRNA表达的抑制率可以达到53%,对蛋白的表达抑制率可以达到92.9%。结论:成功筛选出针对胃癌SGC-7901细胞株VEGF-C的shRNA,为抑制肿瘤淋巴管生成和转移研究提供有效的实验工具。Objective:To screen short hairpin RNA(shRNA)that can interfere with VEGF-C of stomach cancer cell line SGC-7901 effectively.Methods:Two shRNA fragments(VEGFC-1505 and VEGFC-1417)were designed and transfected into stomach cancer cell line SGC-7901 that express VEGF-C positively.The mRNA expression level of VEGF-C was detected with RT-PCR,and the protein of VEGF-C was detected with western-blot.Results:VEGFC-1505 could depress the expression of VEGF-C dramatically,and the inhibition rate of the VEGF-C mRNA re...

关 键 词：RNA干扰技术 胃癌SGC-7901细胞株 VEGF-C 

分 类 号：R735.2[医药卫生—肿瘤]