重组人神经生长因子治疗阿尔茨海默病的机理研究  

The Mechanisms of Recombinant Human Nerve Growth Factor Treatment to Alzheimer's Disease

作　　者：戚其学[1] 曹云鹏[1] 徐楠[2] 

机构地区：[1]中国医科大学附属第一医院神经免疫室 [2]中国医科大学附属第一医院整形外科,辽宁沈阳110001

出　　处：《中国血液流变学杂志》2008年第1期53-55,共3页Chinese Journal of Hemorheology

摘　　要：目的脑内给予重组人神经生长因子(recombinant human nerve growth factor,rhNGF)对阿尔茨海默病(Alzheimer's Disease,AD)模型鼠进行治疗,比较治疗前后动物胆碱乙酰基转移酶(choline acetyltransferase,ChAT)活性变化及神经元的凋亡,探讨NGF治疗AD的作用机理。方法经右侧脑室注射给予AD模型大鼠rhNGF,用液体闪烁仪测定ChAT的活性,TUNEL法检测神经元的凋亡。结果给予rhNGF治疗后,AD鼠额叶、基底节和海马ChAT活性较未治疗组明显升高,神经元凋亡的发生也明显减少。结论rhNGF在AD鼠中发生作用的机理主要是恢复病变区胆碱能神经活性,及减少神经元凋亡。Objective The animal model of Alzheimer s disease(AD) was established, and was treated with nerve growth factor(NGF) to compare choline acetyltransferase(ChAT) activity before and after treatment, and to explore the mechanism of NGF treating AD.Mothods Rat model of AD was established with β-amyloid protein(A β), and NGF was injected by intracerebroventricular. The behavior of rat was observed and ChAT activity was measured with liquid scintillation counter.Results The learning impairment and the memory defi...

关 键 词：阿尔茨海默病 神经生长因子 胆碱乙酰基转移酶 凋亡 

分 类 号：R749.16[医药卫生—神经病学与精神病学]